NKG2D配体在NK细胞杀伤三氧化二砷诱导的KG1a细胞中的作用  

Role of NKG2D ligands in killing KG1a cells treated with arsenic trioxide by NK cells

作　　者：王艳婕 曹威 周彦生 杨瑞[4] 牛新清(指导) WANG Yan-Jie;CAO Wei;ZHOU Yan-Sheng;YANG Rui;NIU Xin-Qing(Second Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院第二附属医院,新乡453003 [2]新郑市第二人民医院,郑州451100 [3]新乡医学院第一附属医院,新乡453003 [4]新乡医学院第三附属医院,新乡453003 [5]新乡医学院医学检验学院,新乡453003

出　　处：《中国免疫学杂志》2021年第3期268-272,共5页Chinese Journal of Immunology

基　　金：河南省自然科学基金(182300410300)。

摘　　要：目的:探讨低浓度的三氧化二砷(ATO)作用急性髓系白血病KG1a细胞株后,对KG1a细胞增殖的影响及与NK细胞联合杀伤肿瘤细胞可能的作用机制。方法:XTT法检测不同浓度ATO对KG1a细胞24 h、48 h的细胞生长抑制作用;LDH检测试剂盒检测定NK细胞对KG1a细胞的杀伤作用;甲基纤维素集落形成实验检测不同实验处理组的集落数;流式细胞术检测低浓度的ATO作用前后KG1a细胞表面NKG2D配体的表达水平。结果:一定浓度范围内ATO对KG1a细胞的增殖抑制作用呈时间浓度依赖性;对比单用ATO处理KG1a细胞组,联合NK细胞后集落形成数明显降低(P<0.05)。对比NKG2D单克隆抗体预处理后的NK细胞联合ATO处理组,NK细胞联用ATO处理组的细胞杀伤率更高、集落形成数明显降低(P<0.05);ATO作用后能够上调KG1a细胞表面NKG2D配体ULBP1的表达(P<0.05)。结论:低浓度的ATO联合NK细胞能抑制KG1a细胞的增殖,其机制可能与低浓度的ATO诱导KG1a细胞表面NKG2D配体ULBP1的表达从而增加NK细胞对KG1a细胞的杀伤作用有关。Objective:To investigate the effect of low concentration of arsenic trioxide(ATO)combined with NK cells on the proliferation of acute myeloid leukemia KG1a cell line and its possible mechanism.Methods:XTT was used to detect the inhibitory effect of different concentrations of ATO on the growth of KG1a cells after 24 h and 48 h.The LDH assay kit was used to detect the killing effect of NK cells on KG1a cells.The formation of colony was detected by methylcellulose colony.The expression of NKG2D ligand in KG1a cells before and after ATO action was detected by cytometry.Results:The inhibitory effect of arsenic trioxide within a certain concentration range on the proliferation of KG1a cells was time-and concentration-dependent.Compared with the group of KG1a cells treated with ATO alone,the number of colonies formed by combining with NK cells was significantly decreased(P<0.05).Compared with the group of NK cells treated with NKG2D monoclonal antibody and ATO treated,NK cells combined with ATO treatment group had higher cell killing rate and lower colony formation number(P<0.05).The expression of NKG2D ligand ULBP1 on KG1a cells was up-regulated after ATO treated(P<0.05).Conclusion:Low concentration ATO combing with NK cells can inhibit the proliferation of KG1a cells,which may be related that the low concentration of ATO reduced the expression of NKG2D ligand ULBP1 on KG1a cells and increased the killing effect of NK cells on KG1a cells.

关 键 词：三氧化二砷 NK细胞 KG1a细胞株 NKG2D配体 

分 类 号：R733.7[医药卫生—肿瘤]