一种多菌灵酶联免疫快速检测方法的建立  

Establishment of arapid detection method for carbendazim enzyme-linked immunosorbent

作　　者：蒋文慧[1] 吴小胜 崔娜 张瑜 彭正学 崔海峰 万宇平 JIANG Wen-hui;WU Xiao-sheng;CUI Na;ZHANG Yu;PENG Zheng-xue;CUI Hai-feng;WAN Yu-ping(Hangzhou People's Procuratorate of Zhejiang Province,Hangzhou,Zhejiang 310012,China;Beijing Kwinbon Biotechnology Company,Beijing 102206,China;Beijing Engineering Research Centre of Food Safety Immunodetection,Beijing 102206,China;Beijing Wanger Biotechnology Company,Beijing 102206,China)

机构地区：[1]浙江省杭州市人民检察院,浙江杭州310012 [2]北京勤邦生物技术有限公司,北京102206 [3]北京市食品安全免疫快速检测工程技术研究中心,北京102206 [4]北京望尔生物技术有限公司,北京102206

出　　处：《食品与机械》2021年第1期94-98,共5页Food and Machinery

基　　金：北京市科技计划课题(编号:Z181100009318006)。

摘　　要：利用酶联免疫吸附法原理建立了一种能够定量测定果蔬、茶叶、烟叶中多菌灵残留量的方法。通过酸碱中和化学反应,制备多菌灵半抗原,再通过免疫小鼠筛选出抗多菌灵单克隆抗体,制得酶标羊抗鼠抗抗体,并将制备出的抗多菌灵单克隆抗体和酶标羊抗鼠抗抗体用于酶联酶联免疫试剂盒的研制。结果表明,在0.1~8.1μg/L的线性范围内,目标物多菌灵线性关系良好,相关系数(R2)可达到0.995。在300,600,1 200μg/kg 3个添加水平下试剂盒的回收率为76.0%~102.2%,相对标准偏差均小于10%。试剂盒检测烟叶、苹果、白菜、茶叶样本中的多菌灵,检测限依次为266.3,421.0,349.1,484.4μg/kg(n=20)。该方法属于快速检测方法,适用于果蔬、茶叶、烟叶中多菌灵残留量的测定。Using the principle of enzyme-linked immunosorbent assay,a method for quantitative determination of carbendazim residues in fruits,vegetables,tea,and tobacco was established.First,the carbendazim hapten was prepared through acid-base neutralization chemical reaction,and then the anti-carbendazim monoclonal antibody was screened out by immunizing mice to prepare the enzyme-labeled goat anti-mouse anti-antibody,and finally the prepared anti-carbendazim Monoclonal antibodies and enzyme-labeled goat anti-mouse anti-antibodies were used in the development of enzyme-linked ELISA kits.The results showed that within the linear range of 0.1~8.1μg/L,the target carbendazim had a good linear relationship,and the correlation coefficient(R~2)could reach 0.995.The recoveries of the kits at the addition levels of 300,600,and 1 200μg/kg were 76.0%~102.2%,and the relative standard deviations were all less than10%.The kit detected carbendazim in tobacco leaves,apples,cabbage,and tea samples,and the detection limits were 266.3,421.0,349.1,and 484.4μg/kg(n=20).This method was a rapid detection method and suitable for the determination of carbendazim residues in fruits,vegetables,tea,and tobacco leaves.

关 键 词：多菌灵 单克隆抗体 ELISA试剂盒 

分 类 号：TS207.5[轻工技术与工程—食品科学]