Nano-ZnO-PDA用于抑制龈下菌斑的体外研究  

In vitro study of Nano-ZnO-PDA for inhibiting subgingival plaque

作　　者：符国富 韩小东[2] 刘族志 林建能 赵永兴 Fu Guofu;Han Xiaodong;Liu Zuzhi;Lin Jianneng;Zhao Yongxing(Quzhou People's Hospital,Hainan Province,Quzhou571700,China;Hainan Branch of PLA General Hospital,Sanya 572013,China)

机构地区：[1]海南省儋州市人民医院,儋州571700 [2]解放军总医院海南分院,三亚572013

出　　处：《广西医科大学学报》2021年第1期90-95,共6页Journal of Guangxi Medical University

基　　金：海南省卫生计生行业科研项目资助(No.16A200075)。

摘　　要：目的:研究一种经聚多巴胺(PDA)改性后的纳米氧化锌(nano-ZnO-PDA)对体外龈下菌斑中细菌的抑制效果,与口腔中常用的普通氧化锌(ZnO)做对比,并探讨nano-ZnO-PDA的生物安全性。方法:利用水浴法合成直径为50 nm左右的球状nano-ZnO(nano-ZnO组),经盐酸多巴胺(DA)在弱碱条件下发生自聚作用,产生PDA包裹在球状nano-ZnO上,形成nano-ZnOPDA(nano-ZnO-PDA组),透射电镜观察改性前、后的纳米结构变化,检测其对金黄色葡萄球菌、绿脓杆菌、牙龈卟啉单胞菌和伴放线聚集杆菌的体外抗菌效果;并与口腔治疗中常用的ZnO(ZnO组)做对比;同时,检测这3种ZnO对人成纤维细胞的毒性以及进行小鼠灌胃实验以进一步验证该材料对小鼠的组织毒性。结果:nano-ZnO以及nano-ZnO-PDA的抗菌效果优于普通ZnO(P<0.05),对于牙龈卟啉单胞菌和伴放线聚集杆菌,nano-ZnO与nano-ZnO-PDA比较,差异有统计学意义(P<0.05)。nano-ZnO-PDA的体外细胞毒性低于普通ZnO和nano-ZnO(P<0.05),3种ZnO对小鼠的体内组织没有产生明显的毒性作用。结论:nano-ZnO-PDA对龈下菌斑中的体外抗菌效果优于普通ZnO,且对人成纤维细胞和小鼠组织并未表现出明显的毒性作用。Objective:To study the inhibition effect of nano-ZnO-PDA modified by polydopamine(PDA)on bacteria in subgingival plaque in vitro,and to compare it with ZnO commonly used in oral cavity,and we discussed the biosafety of this materials.Methods:We synthesised nano-ZnO with a diameter of 50 nm by water bath method.After the self-polymerization of dopamine hydrochloride(DA)under weak alkali condition,PDA was produced and coated on nano-ZnOto form nano-ZnO-PDA.The changes of the nanostructures before and after modification were observed by transmission electron microscopy.The antibacterial effects of nano-ZnO-PDAin vitroon Staphylococcus aureus,Pseudomonas aeruginosa,Porphyromonas gingivalis and Actinomycetes were tested,and compared wirth ZnO,which was commonly used in oral therapy.The toxicity of the three kinds of ZnO materials to human fibroblasts was detected and the intragastric experiment was conducted to further verify the tissue toxicity of these materials to mice.Results:The antibacterial effect of nano-ZnO and nano-ZnO-PDA was better than that of traditional ZnO(P<0.05).Theantibacterial effectson Porphyromonas gingivalis and Actinomyceteswere significantly different between the nano-ZnO and nano-ZnO-PDA(P<0.05).The in vitro cytotoxicity of Nano-ZnO-PDA was lower than that of ZnO and Nano-ZnO(P<0.05).The three kinds of ZnO had no obvious toxic effects on the tissues of mice.Conclusion:The antibacterial effect of nano-ZnO-PDA is better than that of traditional ZnO.The nano-ZnO-PDA shows no obvious toxic effects on human fibroblasts and mouse tissues.

关 键 词：聚多巴胺 表面改性 纳米氧化锌 龈下菌斑 抑菌作用 

分 类 号：R780.2[医药卫生—口腔医学]