3D打印技术在牙髓再生领域的研究进展  

Applications and prospects of 3D printing technology in pulp regeneration

作　　者：谭国忠 江千舟[1] TAN Guozhong;JIANG Qianzhou(Department of Endodontics,Affiliated Stomatology Hospital of Guangzhou Medical University,Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine,Guangzhou 510182,China)

机构地区：[1]广州医科大学附属口腔医院牙体牙髓科·广州市口腔再生医学基础与应用研究重点实验室,广东广州510182

出　　处：《口腔疾病防治》2021年第4期279-283,共5页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：广东省科技计划项目(2018B050502012)。

摘　　要：近年来,牙髓再生成为了口腔医学领域的研究热点,3D打印能实现支架结构和形状的精准调控,为种子细胞黏附和生长因子释放提供基础,通过3D打印组织工程支架构建的3D打印“牙髓复合体”为牙髓再生研究提供了新的方向。本文就3D打印技术应用于牙髓再生的研究作一综述。文献复习结果表明,3D打印“牙髓复合体”中的支架材料、种子细胞和生长因子在牙髓再生的研究中都扮演着重要角色,其中,支架材料能发挥载体作用负载种子细胞和生长因子,并为其提供合适微环境;牙髓干细胞、根尖乳头干细胞和人脱落乳牙牙髓干细胞等常用种子细胞为牙髓再生提供细胞基础;生长因子的引入可以进一步支持牙髓组织的分化和牙髓血管重建,促进牙髓再生。目前,3D打印“牙髓复合体”在牙髓再生的研究中取得了一定进展,在实验室阶段可诱导牙髓样组织的形成,但制备生物活性良好且具有仿生血管和神经效果的3D打印“牙髓复合体”,为根管内细胞提供氧气和营养物质仍是一个巨大的挑战,需进一步的探索和研究。In recent years,pulp regeneration has become a research hotspot in the field of stomatology.3D printing can realize precise control of structure and shape of scaffolds,which provide basis for seed cell adhesion and growth factor release.The 3D printing"pulp complexes"constructed by 3D printing scaffolds for tissue engineering provides a new direction for pulp regeneration research.This paper reviews the applications of 3D printing technology in pulp regeneration.The results of literature review showed that the scaffold materials,seed cells and growth factors in the 3D printing"pulp complexes"all play an important role in the pulp regeneration research.Among them,the scaffold materi⁃als act as carriers to load seed cells and growth factors and provide a suitable microenvironment for them.The common seed cells such as dental pulp stem cells,stem cells from apical papilla and stem cells from the human pulp of exfoliat⁃ed deciduous teeth can provide the cellular basis for pulp regeneration.Moreover,the introduction of growth factors can further support the differentiation of pulp tissue and the reconstruction of pulp vessels and promote pulp regeneration.At present,the 3D printing"pulp complexes"in the study of dental pulp regeneration has made some progress and can induce the formation of pulp⁃like tissues in the laboratory.However,preparing 3D-printing"pulp complex"with good biological activity,which integrates biomimetic blood vessels and nerves to supply oxygen and nutrients to the cells in the root canal,remains a huge challenge and still needs further exploration and research.

关 键 词：牙髓再生 组织工程 3D打印 牙髓复合体 支架材料 种子细胞 生长因子 牙髓血管重建 

分 类 号：R78[医药卫生—口腔医学]