春秋两季油樟叶的油细胞形态、精油成分及其抗氧化活性研究  

Oil Cell Morphology,Essential Oil Constituents and Antioxidant Activity of Cinnamomum longepaniculatum Leaves Harvested in Spring and Autumn

作　　者：程贤[1] 毕良武[1] 李胜男 陈玉湘[1] 赵振东[1] 莫开林[2] CHENG Xian;BI Liangwu;LI Shengnan;CHEN Yuxiang;ZHAO Zhendong;MO Kailin(Institute of Chemical Industry of Forest Products,CAF,National Engineering Lab.for Biomass Chemical Utilization,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,Key Lab.of Biomass Energy and Material,Jiangsu Province,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China;Sichuan Academy of Forestry Sciences,Chengdu 610081,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点实验室,江苏省生物质能源与材料重点实验室,江苏省林业资源高效加工利用协同创新中心,江苏南京210042 [2]四川省林业科学研究院,四川成都610081

出　　处：《林产化学与工业》2021年第1期38-44,共7页Chemistry and Industry of Forest Products

基　　金：四川省院省校合作项目(2019YFSY0002);中国林科院林产化学工业研究所研究团队建设创新工程项目(LHSXKQ2)。

摘　　要：首先利用组织透明法对春秋两季油樟叶中油细胞进行形态观察,统计油细胞密度和直径,利用扫描电镜(SEM)进一步观察油细胞的细胞壁结构;其次对春秋两季油樟叶进行油樟精油的提取,利用GC-MS鉴定油樟精油的化学成分,利用GC分析了1,8-桉叶素、α-松油醇和γ-松油烯3种主要成分的含量;最后对油樟精油的抗氧化活性进行检测。研究结果表明:春季和秋季油樟叶片的油细胞密度分别为(41±3)和(38±2)个/mm^2,油细胞直径分别为(51.87±1.64)和(36.89±2.64)μm,细胞壁厚度分别为1.65和0.63μm;春季和秋季油樟叶的精油提取得率分别为4.45%和3.09%(以干质量计),GC-MS表征鉴定出16个化合物,3种主成分的含量没有显著性差异;春秋两季的油樟精油总抗氧化能力分别为6.50和5.50U/mL,达到Vc的40%,清除DPPH自由基的半数抑制质量浓度(IC50)均为0.2g/L。The tissue transparency method was used to observe the morphology of oil cells in the leaves of Cinnamomum longepaniculatum(Gamble)N.Chao ex H.W.Li in spring and autumn,and the density and diameter of the oil cells were determined.Cell wall structure of the oil cells was observed with scanning electron microscopy.GC-MS was used to characterize the chemical composition of the essential oils extracted from the leaves of C.longepaniculatum in in spring and autumn.Eucalyptol,α-terpineol,andγ-terpinene content was analyzed by GC.Finally,antioxidant activity of the essential oils was evaluated.The density of oil cells of C.longepaniculatum leaves in spring and autumn was(41±3)and(38±2)per mm^2,the diameter was(51.87±1.64)and(36.89±2.64)μm,and the cell wall thickness was 1.65 and 0.63μm,respectively.The extraction yields of essential oils from C.longepaniculatum leaves were 4.45%and 3.09%(based on dry weight)in spring and autumn,respectively.Sixteen types of chemical compounds were identified with GC-MS,and no significant difference was observed in the content of the three main components.The total antioxidant capacity of the essential oils from C.longepaniculatum leaves harvested in spring and autumn was 6.50 and 5.50 U/mL,respectively(40%of Vc),and the IC 50 value corresponding to scavenging of DPPH free radicals was 0.2 g/L.

关 键 词：油樟 油细胞 精油 抗氧化活性 

分 类 号：TQ35[化学工程]