Waterlogging tolerance and wood properties of transgenic Populus alba×glandulosa expressing Vitreoscilla hemoglobin gene(Vgb)  

作　　者：Yiliang Li Weixi Zhang Wenxu Zhu Bingyu Zhang Qinjun Huang Xiaohua Su 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China [2]Key Laboratory of Tree Breeding and Cultivation,National Forestry and Grassland Administration,Beijing 100091,People’s Republic of China [3]Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520,People’s Republic of China [4]Guangdong Academy of Forestry,Guangzhou 510520,People’s Republic of China [5]College of Forestry,Shenyang Agricultural University,120 Dongling Street,Shengyang 110866,People’s Republic of China

出　　处：《Journal of Forestry Research》2021年第2期831-839,共9页林业研究（英文版）

基　　金：supported by the Basic Research Fund of RIF(Grant No.CAFYBB2017ZA001-3);the National Natural Science Foundation of China(Grant No.31700589);the Forestry Genetic Breeding National Key Laboratory(Chinese Academy of Forestry Sciences)Open Project(Grant No.TGB 2013005).

摘　　要：Because overexpression of Vitreoscilla hemoglobin gene(Vgb)gene in plants can enhance tolerance to waterlogging,here Vgb was inserted into Populus alba×glandulosa to investigate its expression and effects on growth and physiological responses to waterlogging stress in the transgenic poplars.Southern blotting and RT-PCR analysis of Vgb-transgenic P.alba×glandulosa showed that the Vgb gene was integrated into the genome of the V13-81 and V13-85 transgenic lines and expressed.In greenhouse waterlogging stress tests,mortality of the transgenic poplar was significant lower than that of nontransgenic plants with increasing treatment time from 2 to 22 days.The transgenic plants had higher chlorophyll content and less chloroplast damage than in the control plants.Additionally,starch accumulation increased,and growth was enhanced in the transgenic plants,suggesting that the Vgb-expressing lines had improved energy reserves.Field trials of the transgenic poplar suggested that Vgb expression promotes growth and influences wood quality.Taken together,our results suggest that the expression of Vgb can increase the accumulation of chlorophyll and starch in the transgenic poplar,improve its ability to endure flooding,and improve growth and wood quality of the transgenic plants.

关 键 词：Populus alba×glandulosa vgb gene Waterlogging stress GROWTH MICROSCOPY 

分 类 号：S792.11[农业科学—林木遗传育种]