炎症性肠病患者血清miR-181a-5p和miR-126表达水平及其与肠道菌群相关性的研究  

Expressions of miR-181a-5p and miR-126 in the serum of patients with inflammatory bowel disease and their correlation with intestinal flora

作　　者：崔昭[1] 梁博[1] 李国东 李会荣[1] CUI Zhao;LIANG Bo;LI Huirong;LI Guodong(Dermatology Laboratory,Baoding Second Hospital,Baoding 071000,China;Department of Digestion,Baoding First Central Hospital,Baoding 071000,China)

机构地区：[1]保定市第二医院皮肤科实验室,071000 [2]保定市第一中心医院消化三科

出　　处：《国际消化病杂志》2021年第1期33-37,共5页International Journal of Digestive Diseases

摘　　要：目的探讨miR-181a-5p、miR-126在炎症性肠病(IBD)患者血清中的表达及其与肠道菌群的相关性。方法收集2016年10月至2018年12月保定市第二医院收治的100例活动期IBD患者作为研究组,根据IBD类型分为溃疡性肠炎(UC)组(60例)和克罗恩病(CD)组(40例),另选择同期在本院体检的50名健康志愿者作为对照组。采用实时荧光定量聚合酶链反应(Real-time qPCR)检测血清miR-181a-5p、miR-126的表达水平,根据检测结果将IBD患者分为miR-181a-5p高表达组(70例)和低表达组(30例)、miR-126高表达组(80例)和低表达组(20例)。对各组的肠道菌群进行培养分析。采用Pearson法分析IBD患者血清miR-181a-5p、miR-126表达水平与肠道菌群数量的相关性。结果与对照组相比,研究组、UC组及CD组的血清miR-181a-5p、miR-126的相对表达量均显著升高(P均<0.05)。与对照组相比,UC组肠球菌属、酵母菌属、拟杆菌属、双歧杆菌属、消化球菌属、乳杆菌属、小梭菌属数量显著增多(P<0.05),CD组肠球菌属、酵母菌属、拟杆菌属、双歧杆菌属、消化球菌属、乳杆菌属数量显著增多(P<0.05)。与对照组相比,UC组拟杆菌属、双歧杆菌属、消化球菌属、乳杆菌属的培养阳性率显著升高(P<0.05);与对照组相比,CD组酵母菌属、双歧杆菌属、消化球菌属、乳杆菌属的培养阳性率显著升高(P<0.05)。与miR-181a-5p低表达组相比,miR-181a-5p高表达组肠球菌属、酵母菌属、葡萄球菌属的数量显著增多(P<0.05);与miR-126低表达组相比,miR-126高表达组肠球菌属、双歧杆菌属、消化球菌属、葡萄球菌属的数量显著增多(P<0.05)。miR-181a-5p表达水平与肠球菌属、酵母菌属、双歧杆菌属、肠杆菌属呈负相关(P<0.05),miR-126表达水平与消化球菌属、葡萄球菌属呈负相关(P<0.05)。结论IBD患者的血清miR-181a-5p、miR-126表达水平升高,两者与IBD患者肠道内肠球菌属、酵母菌属等菌群组成变化密�Objective This paper intends to explore the expressions of miR-181a-5p and miR-126 in the serum of patients with inflammatory bowel disease(IBD)and their correlation with intestinal flora.Methods A total of 100 patients with active IBD admitted to Baoding Second Hospital from October 2016 to December 2018 were assigned to the ulcerative enteritis(UC)group(n=60)and the Crohn′s disease(CD)group(n=40)based on the types of IBD,while another 50 healthy volunteers who had a physical examination in the hospital during the same period were assigned to the control group.Real-time qPCR was used to detect the expressions of serum miR-181a-5p and miR-126,and patients with IBD were divided into miR-181a-5p high expression groups(n=70)and miR-181a-5p low expression group(n=30),miR-126 high expression group(n=80)and miR-126 high low expression group(n=20).The intestinal flora of each group was cultured and analyzed.The Pearson method was used to analyze the correlation between the expressions of miR-181a-5p and miR-126 in the serum and the number of flora in IBD patients.Results Compared with the control group,the expressions of miR-181a-5p and miR-126 in the serum of the study group,the UC group,and the CD group were significantly increased(all P<0.05).Compared with the control group,the numbers of Enterococcus,Saccharomyces,Bacteroides,Bifidobacterium,Peptococcus,Lactobacillus,and Clostridium were significantly increased in the UC group(P<0.05).Besides,the numbers of genus Bacteroides,Bifidobacterium,Peptococcus,and Lactobacillus increased significantly(P<0.05).Compared with the control group,the culture-positive rates of Bacteroides,Bifidobacterium,Peptococcus,and Lactobacillus in the UC group were significantly increased(P<0.05).Compared with the control group,the culture-positive rates of Saccharomyces,Bifidobacteria,Peptococcus,and Lactobacillus in the CD group were significantly increased(P<0.05).Compared with the miR-181a-5p low expression group,the numbers of Enterococcus,Saccharomyces,Staphylococcus in the miR-181a-

关 键 词：miR-181a-5p MIR-126 炎症性肠病 肠道菌群 

分 类 号：R574[医药卫生—消化系统]