SERS技术对生鲜猪肉中β-受体激动剂的快速检测研究  

SERS for Rapid Detection of β-Agonist in Fresh Pork

作　　者：肖雄枫 宋移欢 杨昌彪 李占彬 曹俊杰 XIAO Xiongfeng;SONG Yihuan;YANG Changbiao;LI Zhanbin;CAO Junjie(Guizhou Institute of Analysis and Test,Guiyang 550002,China;College of Public Health,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州省分析测试研究院,贵州贵阳550002 [2]贵州医科大学公共卫生学院,责州贵阳550025

出　　处：《食品科技》2020年第12期318-323,共6页Food Science and Technology

基　　金：贵阳国家高新技术产业开发区管理委员会科技计划项目(GXYF-2017-004)。

摘　　要：目的:采用表面增强拉曼光谱法(Surface-enhanced raman spectroscopy,简称SERS)与快速溶剂提取前处理技术,建立生鲜猪肉中β-受体激动剂的检测方法。方法:采用SERS法与快速溶剂提取前处理技术,对猪肉中克伦特罗、莱克多巴胺、喷布特罗、西马特罗进行检测,在碱性条件下使用乙酸乙酯提取目标物,再以0.1 mol/L盐酸溶液萃取净化,以去除基质中脂肪、蛋白质等大分子物质的干扰。将采集的光谱进行降噪、平滑、"Savitzky-Golay"二阶导数等处理后分析。结果:4种β-受体激动剂的各自特征峰在一定浓度范围内存在良好的线性关系;配制3个浓度样本验证方法准确度,平均回收率为85.2%~101.5%,相对标准偏差(RSD)为2.3%~7.9%,检出限低至0.005 μg/g,定量限为0.05 μg/g。结论:该方法对猪肉中β-受体激动剂的检测具有良好的效果,步骤简单、周期短、成本低,在动物食品检测流通环节具有广阔的应用价值。Objective:Using surface-enhanced raman spectroscopy and rapid solvent extraction pretreatment technology to establish a method for detecting β-agonist in fresh pork.Methods:SERS method and rapid solvent extraction pretreatment technology were used to detect clenbuterol,ractopamine,penbuterol and cimaterol in pork.Under alkaline conditions,ethyl acetate is used to extract the target,and then extracted and purified with 0.1 mol/L hydrochloric acid solution to remove the interference of macromolecules such as fat and protein in the matrix.The collected spectrum was analyzed after noise reduction,smoothing,and "Savitzky-Golay" second derivative processing.Results:The characteristic peaks of the four β-agonists have a good linear relationship within a certain concentration range;preparing three concentration samples to verify the accuracy of the method,the average recovery rate is 85.2%~101.5%,and the RSD is 2.3%~7.9%,the detection limit is as low as 0.005 μg/g,and the limit of quantification is 0.05 μg/g.Conclusion:The method has a good effect on the detection of β-agonist in pork,with simple steps,short cycle and low cost,and has broad application value in the circulation of animal food testing.

关 键 词：SERS 金溶胶 β-受体激动剂 猪肉 

分 类 号：O657.37[理学—分析化学] TS207.5+3[理学—化学]