大孔吸附树脂-高速逆流色谱法分离纯化苦水玫瑰花渣中的鞣花酸  

Separation and Purification of Ellagic Acid from Rosa setate x Rosa rugosa Waste by Macroporous Resin and High-speed Countercurrent Chromatography

作　　者：吴梦琪 黄海 蔡狄宏 徐志珍[1] 夏玮[1] 张文清[1] WU Mengqi;HUANG Hai;CAI Dihong;XU Zhizhen;XIA Wei;ZHANG Wenqing(School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学化学与分子工程学院,上海200237

出　　处：《食品工业科技》2021年第5期182-187,共6页Science and Technology of Food Industry

摘　　要：研究从苦水玫瑰花渣中分离纯化鞣花酸的方法及其抗氧化活性。玫瑰花渣粗提物先经过大孔吸附树脂初步分离,富集目标组分。然后以正己烷-乙酸乙酯-甲醇-水(2∶7∶3.5∶7,v/v/v/v)溶剂体系的上相做固定相,下相做流动相,在转速850 r·min^(-1)、流动相体积流量3 mL·min^(-1)、温度为30℃、进样量800 mg条件下,分离得到鞣花酸,纯度为96.2%。体外抗氧化测定结果显示其对DPPH·和ABTS^(+)·清除作用较强,IC_(50)值分别为(2.54±0.05)、(9.62±0.17)mg·L^(-1);总抗氧化能力FRAP值为(180.85±3.16)μmol·L^(-1)·mg^(-1)。该分离技术方法简便,产物纯度高,为苦水玫瑰花渣的综合利用提供了理论依据。A purification method of ellagic acid(Ea)from Kushui rose waste and its antioxidant activities were studied.Firstly,the crude alcohol extracts of rose residues were purified by macroporous adsorption resin to enrich the target component.Then,the separation was performed using high-speed count-current chromatography with a two-phase solvent system consisting of n-hexane-ethyl acetate-methanol-water(2∶7∶3.5∶7,v/v/v/v).Ellagic acid with purity of 96.2%was achieved with the flow rate of 3.0 mL·min^(-1),the speed of 850 r·min^(-1),the system temperature of 30℃and sample loading of 800 mg,upper phase as stationary phase and lower phase as mobile phase.Furthermore,the in-vitro antioxidant test results showed that Ea possessed the strong antioxidant effects on the clearance of DPPH·and ABTS^(+)·,with IC 50 values of(2.54±0.05)and(9.62±0.17)mg·L^(-1),respectively.Meanwhile,the total antioxidant capacity FRAP value was(180.85±3.16)μmol·L^(-1)·mg^(-1).This simple technology obtained the products with high purity,which provided a theoretical basis for the integrated utilization of rose waste biomass.

关 键 词：苦水玫瑰 花渣 鞣花酸 大孔吸附树脂 高速逆流色谱 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]