胆管细胞型肝癌lncRNA-mRNA共表达网络的高通量基因芯片筛查分析  

High throughput microarray analysis of Lncrna-mRNA co-expression network in intrahepatic cholangiocarcinoma

作　　者：唐津天[1] 王瑞宾 王伯庆[1] TANG Jintian;WANG Ruibin;WANG Boqing(Hepatobiliary and Pancreatic Surgery,Affiliated Cancer Hospital,Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830000,China;the Third Clinical Medical College,Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属肿瘤医院肝胆胰外科,新疆乌鲁木齐830000 [2]新疆医科大学第三临床医学院,新疆乌鲁木齐830000

出　　处：《中国医药导报》2021年第3期21-25,F0004,共6页China Medical Herald

基　　金：新疆维吾尔自治区自然科学基金项目(2016D01 C374)。

摘　　要：目的探索胆管细胞型肝癌的长链非编码RNA(lncRNA)-信使RNA(mRNA)共表达网络,并对差异表达的lncRNA进行功能分析。方法选取2017年1月—6月在新疆医科大学附属肿瘤医院肝胆外科手术切除的5例胆管细胞型肝癌新鲜组织标本。采用高通量芯片技术对5例胆管细胞型肝癌组织进行mRNA和lncRNA差异表达谱分析,构建lncRNA-mRNA共表达网络。选择显著差异的Top1 lncRNA进行mRNA共表达筛查,进行GO分析和Pathway分析。并对Top1 lncRNA及其共表达mRNA在其他30例胆管细胞型肝癌标本中进行验证。结果在筛选阈值为P≤0.05,log2|差异倍数|≥1(FC)的情况下,获得差异基因共计475个,其中上调mRNA 213个,下调mRNA 262个;获得差异lncRNA共计438个,其中上调lncRNA131个,下调lncRNA307个。筛选获得Top1 lncRNA为氨基甲酰磷酸合成酶Ⅰ-内含子转录本1(CSP1-IT1)。GO分析和Pathway分析结果示CSP1-IT1与20个生物过程和2个细胞组成功能相关,涉及8个细胞信号通路。结论胆管细胞型肝癌中存在的mRNA和lncRNA形成复杂的共表达网络,参与多个重要的生物学过程。Objective To explore the co-expression network of long non coding RNA(lncRNA)-messenger RNA(mRNA)in intrahepatic cholangiocarcinoma,and to analyze the function of the differentially expressed lncrnas.Methods From January to June 2017,fresh tissue sample of five patients with cholangiocarcinoma who were resected by hepatobiliary surgery in the Affiliated Cancer Hospital,Xinjiang Medical University were selected.High throughput microarray technology was used to analyze the mRNA and lncRNA differential expression profiles,and to construct the lncRNA-mRNA co-expression network.Top 1 lncRNA with significant difference was selected for mRNA co-expression screening,GO analysis and Pathway analysis.In addition,Top1 lncRNA and its co-expression mRNA were verified in other 30 specimens of intrahepatic cholangiocarcinoma.Results When the screening threshold was P≤0.05 and log2|Fold Change(FC)|≥1,475 differential genes were obtained,including 213 up-regulated mRNA and 262 down regulated mRNA;438 differential lncRNA were obtained,including 131 up-regulated lncRNA and 307 down regulated lncRNA.Top1 lncRNA was screened as carbamoyl phosphate synthase I-intron transcript 1(CPS1-IT1).GO analysis and Pathway analysis showed that CPS1-IT1 was associated with 20 biological processes and two cellular functions,involving eight cell signaling pathways.Conclution The mRNA and lncRNA in intrahepatic cholangiocarcinoma form a complex co-expression network and participate in many important biological processes.

关 键 词：胆管细胞型肝癌 共表达网络 基因芯片 长链非编码RNA 

分 类 号：R735.7[医药卫生—肿瘤]