蔗糖水解制取结晶果糖技术研究  

Study on the Optimum Technology of Producing Crystalline Fructose by Hydrolysis of Sucrose

作　　者：胡瑞云[1] 王智能[1] 杨柳 杨婷 应雄美[1] 郭家文[1] 沈石妍 李艳芳[1] HU Rui-yun;WANG Zhi-neng;YANG Liu;YANG Ting;YING Xiong-mei;GUO Jia-wen;SHEN Shi-yan;LI Yan-fang(Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement of Yunnan,Kaiyuan,Yunnan 661699)

机构地区：[1]云南省农业科学院甘蔗研究所云南省甘蔗遗传改良重点实验室,云南开远661699

出　　处：《甘蔗糖业》2021年第1期74-81,共8页Sugarcane and Canesugar

基　　金：云南省重大科技专项-甘蔗所现代科研所建设(2019ZG012-01)资助。

摘　　要：为提升制糖产业的综合效益,以白砂糖为原料开发附加值更高的结晶果糖,探讨用蔗糖原料制取高纯度结晶果糖的最优工艺方法。实验以高浓度蔗糖液(55%w/w)为原料,食品酸味剂柠檬酸为水解剂,通过蔗糖水解、脱色、阴阳离子树脂除盐,以钙型树脂分离、纯化果葡糖液,在常温下得到结晶果糖和结晶葡萄糖。实验优化蔗糖最佳水解工艺条件:水解温度80℃,水解时间4.0 h,柠檬酸用量0.50%,蔗糖水解率为100%;果糖结晶工艺条件:在水-乙醇比例为1∶4的体系下,果糖纯度88%以上、糖膏固形物含量87°Bx以上、晶种用量为糖膏总量5%、养晶时间48 h,果糖结晶率达到56.34%;葡萄糖结晶工艺条件:在水-乙醇比例为1∶2的体系下,葡萄糖纯度65%以上、糖膏固形物含量83°Bx以上、晶种用量为糖膏总量5%、养晶时间48 h,葡萄糖结晶率达到63.27%。实验选择高浓度蔗糖液水解及常温下结晶的方式,可降低能源消耗,同时通过将纯度65%以上的葡萄糖液直接结晶分离,使母液中果糖纯度从33.3%提升至60.2%,起到了分离纯化果糖液的效果,有效减轻果葡糖液的分离负荷。In order to improve the comprehensive benefits of sugar industry,the crystal fructose with higher added value is developed with white granulated sugar as raw material,and the optimal process for producing high purity crystalline fructose from sucrose is discussed.High concentration sucrose solution(55%w/w)is used as raw material and citric acid as sucrose hydrolysate.Fructose is separated and purified by calcium resin through sucrose hydrolysis,decolorization and Cation Anion Exchange.The optimal hydrolysis conditions of sucrose are as follows:The results show that hydrolysis temperature is 80℃,hydrolysis time is 4.0 h,citric acid dosage is 0.50%,and sucrose hydrolysis rate is 100%;The conditions of fructose crystallization are as follows:under the system of water ethanol ratio of 1∶4,the purity of fructose is more than 88%,the solid content of sugar paste is more than 87°Bx,the amount of seed crystal is 5%and the crystallization time is 48 h.The crystallization rate of fructose is 56.34%;The conditions of glucose crystallization are as follows:in the system of water ethanol ratio of 1∶2,the purity of glucose is above 65%,the solid content of sugar paste is more than 83°Bx,the amount of crystal seed is 5%and the crystallization time is 48 h.The crystallization rate of glucose reaches 63.27%.The method of high concentration sucrose hydrolysis and crystallization at room temperature can reduce energy consumption.At the same time,the purity of fructose in mother liquor is increased from 33.3%to 60.2%by direct crystallization and separation of glucose solution with purity above 65%.This method could reduce the effect of separation and purification of fructose solution and load of fructose solution.

关 键 词：蔗糖 果糖 葡萄糖 水解 分离 结晶 

分 类 号：TS245.4[轻工技术与工程—制糖工程]