肝癌患者组织中Cyclin E、p27kip1和Ki-67蛋白的表达与临床特征的相关性  

Expressions of Cyclin E,p27kip1 and Ki-67 proteins in liver cancer tissues and their relationship with clinical characteristics

作　　者：王杉 梁鑫鸿 朱庚 李炎铭 WANG Shan;LIANG Xinhong;ZHU Geng(Department of General Surgery,Hangzhou Hospital,Zhejiang Medical and Health Group,Hangzhou 310000,China)

机构地区：[1]浙江省医疗健康集团杭州医院普外科,浙江杭州310000

出　　处：《全科医学临床与教育》2021年第2期113-116,120,共5页Clinical Education of General Practice

摘　　要：目的分析肝癌患者组织细胞周期素E(Cyclin E)、抑制因子p27kip1和核抗原-67(Ki-67)基因和蛋白的表达及与临床特征的关系。方法选择肝癌患者共90例(观察组),同期选择50例肝脏血管瘤患者(对照组),采用免疫组化染色法检测观察组肿瘤组织和癌旁正常组织与对照组的Cyclin E、p27kip1和Ki-67蛋白阳性表达,反转录PCR(RT-PCR)法检测组织Cyclin E、p27kip1和Ki-67 mRNAs表达水平,分析其与患者和肿瘤临床特征的关系。结果观察组肿瘤组织Cyclin E和Ki-67阳性表达率及mRNAs水平比癌旁组织和对照组增加,p27kip1阳性表达率及mRNA水平比癌旁组织和对照组降低(χ~2分别=33.91、23.77;36.44、23.17;-16.36、-7.81;t分别=20.40、17.47;11.26、9.69;-14.16、-14.84,P均<0.05)。观察组肿瘤组织Cyclin E、Ki-67和p27kip1蛋白阳性表达与患者性别、年龄、肿瘤直径无关(χ~2分别=0.64、0.18、0.51;0.49、0.17、0.62;0.02、1.44、0.86,P均>0.05),但与肿瘤TNM分期、血液或淋巴转移、肿瘤分化级别密切相关(χ~2分别=19.00、7.32、10.40;10.79、7.79、9.37;15.26、6.58、6.67,P均<0.05)。结论肝癌的发生和发展与组织Cyclin E、p27kip1和Ki-67蛋白的表达异常密切相关。Objective To analyze expressions of Cyclin E,inhibitor p27kip1 and nuclear antigen-67 genes(Ki-67)and proteins in liver cancer tissues and their relationship to clinical characteristics.Methods Totally 90 patients with hepatocellular carcinoma(observation group)and 50 patients with hepatic hemangioma(control group)were selected.The Cyclin E,p27kip1 and Ki-67 proteins were detected by immunohistochemical staining in tumor tissues and adjacent tissues of observation group and control group.The expressions of Cyclin E,p27kip1 and Ki-67 mRNAs were detected by RT-PCR,and the relationships between expressions of Cyclin E,p27kip1,Ki-67 and patients’clinical features were analyzed.Results The positive expression rates and mRNAs levels of Cyclin E and Ki-67 of tumor tissues in observation group were significantly higher than adjacent tissues in observation group and control group,while positive expression rate and mRNA level of p27kip1 of tumor tissues were lower than adjacent tissues in observation group and control group(χ2=33.91,23.77,36.44,23.17,-16.36,-7.81,t=20.40,17.47,11.26,9.69,-14.16,-14.84,P<0.05).The positive expressions of Cyclin E,Ki-67 andp 27kip1 proteins of tumor tissues in observation group were not related to patients’sex,age and tumor diameter(χ2=0.64,0.18,0.51,0.49,0.17,0.62,0.02,1.44,0.86,P>0.05),while were close related to TNM staging,blood or lymphatic metastasis,and differentiation of tumors(χ2=19.00,7.32,10.40,10.79,7.79,9.37,15.26,6.58,6.67,P<0.05).Conclusion The occurrence and development of hepatocellular carcinoma are closely related to abnormal expressions of tissues Cyclin E,p27kip1 and Ki-67 proteins.

关 键 词：肝癌 细胞周期素E 抑制因子p27kip1 核抗原-67 

分 类 号：R735.7[医药卫生—肿瘤]