慢性HBV感染患者TIM-3基因多态性及其与抗病毒治疗疗效关系  

Correlation between TIM-3 gene polymorphism and efficacy of antiviral therapy in patients with chronic HBV infection

作　　者：常文娟[1] 王慧[1] 张艳鹤[1] 冯卫华[1] 杨记康 CHANG Wen-juan;WANG Hui;ZHANG Yan-he;FENG Wei-hua;YANG Ji-kang(Jiaozuo People's Hospital,Jiaozuo,Henan 454000,China)

机构地区：[1]焦作市人民医院消化二区,河南焦作454000

出　　处：《中华医院感染学杂志》2021年第2期176-179,共4页Chinese Journal of Nosocomiology

基　　金：河南省科技攻关基金资助项目(9412013Y0249)。

摘　　要：目的分析慢性乙型肝炎病毒(HBV)感染患者T细胞免疫球蛋白域粘蛋白域蛋白-3(TIM-3)基因多态性及其与抗病毒治疗的疗效关系。方法选取焦作市人民医院消化二区2017年1月-2018年6月296例慢性HBV感染患者为研究对象,其中乙肝164例(乙肝组),肝硬化83例(肝硬化组),肝细胞癌49例(肝癌组),并纳入32名同期健康体检者作为对照组;比较入组研究对象外周血单核细胞TIM-3、谷丙转氨酶(ALT)、HBV脱氧核糖核酸(DNA)表达水平;并将乙肝组按照疗效分为疗效较好组及疗效较差组,比较治疗前后TIM-3表达水平及TIM-3基因单核苷酸多态性(SNP)位点基因型及等位基因频率。结果四组外周血单核细胞TIM-3及ALT表达水平比较差异有统计学意义(P<0.05),对照组表达水平最低,肝癌组表达水平最高(P<0.05);肝硬化组、肝癌组HBV DNA水平比较差异无统计学意义,但均低于乙肝组(P<0.05);乙肝疗效较好组治疗前后外周血单核细胞TIM-3表达水平均低于疗效较差组(P<0.05);乙肝组不同疗效患者rs11741184基因型CC、CG、GG分布频率及等位基因C、G分布频率和rs13170556基因型AA、GA、GG分布频率及等位基因A、G分布频率比较,差异均无统计学意义。结论外周血单核细胞TIM-3表达水平与慢性HBV感染诊疗过程中肝损害情况密切相关,但TIM-3基因多态性在慢性HBV感染的调控作用有待进一步探究。OBJECTIVE To analyze the correlation between gene polymorphism of T cell immunoglobulin domain and mucin domain protein-3(TIM-3) and efficacy of antiviral therapy in patients with chronic hepatitis B virus(HBV) infection. METHODS Totally 296 patients with chronic HBV infection who were treated in the second department of gastroenterology of Jiaozuo People′s Hospital from Jan 2017 to Jun 2018 were enrolled in the study, including 164 cases of hepatitis B(the hepatitis B group), 83 cases of liver cirrhosis(the cirrhosis group) and 49 cases of hepatocellular carcinoma(the hepatoma group);meanwhile, 32 healthy people who received physical examination were set as the control group.The levels of peripheral blood mononuclear cell TIM-3, alanine aminotransferase(ALT) and HBV deoxyribonucleic acid(DNA) were compared among the study objects.The patients in the hepatitis B group were divided into the good efficacy group and the poor efficacy group according to the efficacy.The level of peripheral blood mononuclear cell TIM-3, genotypes of TIM-3 single nucleotide polymorphism(SNP) locus and allele frequency were compared before and after the treatment. RESULTS There were significant differences in the levels of peripheral blood mononuclear cell TIM-3 and ALT among the four groups(P<0.05), the levels of above indexes of the control group were the lowest, and the levels of above indexes of the hepatoma group were the highest(P<0.05).There was no significant difference in the HBV-DNA level between the cirrhosis group and the hepatoma group, however, the HBV-DNA level of the two groups was significantly lower than that of the hepatitis B group(P<0.05).The peripheral blood mononuclear cell TIM-3 level of the good efficacy group was significantly lower than that of the poor efficacy group before and after the treatment(P<0.05).There were no significant differences in the distribution frequencies of rs11741184 genotypes CC, CG and GG, alleles C and G, rs13170556 genotypes AA, GA and GG, and alleles A and G between the two groups

关 键 词：乙型肝炎病毒 慢性 T细胞免疫球蛋白域粘蛋白域蛋白-3 基因多态性 疗效 谷丙转氨酶 

分 类 号：R735.7[医药卫生—肿瘤]