水产品中孔雀石绿、硝基呋喃、氯霉素残留免疫胶体金前处理过程的探讨  

Discussion on the pretreatment of residuals of malachite green,nitrofuran and chloramphenicol in aquatic products by quick inspect of immune colloidal gold technique

作　　者：叶江雷[1] 林功师 林楠 林涛 YE Jianglei;LIN Gongshi;LIN Nan;LIN Tao(Xiamen Ocean Vocational College,Xiamen 361100,China;Xiamen Marine&Fisheries Research Institute,Xiamen 361008,China;Fujian Fisheries Technology Extension Center,Fuzhou 350002,China)

机构地区：[1]厦门海洋职业技术学院,福建厦门361100 [2]厦门市海洋与渔业研究所,福建厦门361008 [3]福建省水产技术推广总站,福建福州350002

出　　处：《渔业研究》2021年第1期73-79,共7页Journal of Fisheries Research

基　　金：厦门市海洋与渔业发展专项资金(19CZB015HJ02).

摘　　要：国家明令禁止孔雀石绿、硝基呋喃类、氯霉素等三类六项药物在食品动物中使用,在水产品中不得检出。当前,这三类药残的快速检测以免疫胶体金法为主,当遇到较为特殊的样品(特别是含脂量高)时,检测的准确率降低,易出现“假阴性”的现象。本文在梳理出不同前处理方法的基础上,给出提高检测准确率的建议。It was banned by the state,that the residues of 6 kind of drugs,including malachite green,nitrofuran(furazolidone,furaltadone,nitrofurazone,nitrofurantoin),and chloramphenicol could not be detected in aquatic products.At present,the quick inspect of these residues was mainly based on immune colloidal gold technique.The accuracy of inspecting was reduced,as high fat samples,and the probability of“false negative”was arised.Commercialized quick inspect kits usually have certain pertinence.The basic inspecting unit usually select a certain type of kit that could cover the range to meet the daily inspect;however,in case of abnormal samples,the improvement of the pretreatment must be made by the kit supplier.This situation may be changed,on the basis of discussing the pretreatment process of these three kinds of residues in aquatic products in recent years.In this paper,we provided clues,including various interference factors and corresponding reagents.And tied to make it possible,that those basic level inspectors could be optimized or even designed the pretreatment by themselves,according to the method of the daily inspecting.There were many kinds of aquatic products,and the matrix of samples was quite different.Even if the authors had made improvements in some aspects for a certain type of kit,they could not completely cover all samples.It was important to enabled the readers could properly optimize the pre-treatment on the basis of the adopted method,when encountering abnormal samples.The optimized method verification and process recording should be made for future checking and item expansion.

关 键 词：前处理 免疫胶体金 孔雀石绿 硝基呋喃类 氯霉素 水产品 

分 类 号：O652.6[理学—分析化学] S942[理学—化学]