番茄叶片及果实总RNA提取方法的比较  

Comparison of RNA extraction methods from tomato leaves and fruits

作　　者：郭荔雯 摆福红 沙晓东 张风琴 王林[1] 王晓敏[1,2,3,4] GUO Li-wen;BAI Fu-hong;SHA Xiao-dong;ZHANG Feng-qin;WANG Lin;WANG Xiao-min(School of Agriculture,Ningxia University,Yinchuan 750021,China;Ningxia Facility Horticulture(Ningxia University)Technology Innovation Center,Yinchuan 750021,China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan 750021,China;Ningxia Modern Facility Horticulture Engineering Technology Center,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏设施园艺(宁夏大学)技术创新中心,银川750021 [3]宁夏优势特色作物现代分子育种重点实验室,银川750021 [4]宁夏现代设施园艺工程技术研究中心,银川750021

出　　处：《湖北农业科学》2021年第3期141-144,共4页Hubei Agricultural Sciences

基　　金：宁夏回族自治区级大学生创新创业训练计划项目(201810749094);2016年度教育部春晖计划项目。

摘　　要：以番茄品种Moneymaker(MM)为材料,比较了试剂盒法、Trizol法和Trizol改良法3种方法对番茄叶片及果实不同时期总RNA的提取效果,分别采用核酸蛋白检测仪及琼脂糖凝胶电泳,测定了提取样本总RNA的浓度、纯度及完整性。结果表明,就番茄叶片而言,3种方法提取的总RNA纯度均较好;其中Trizol改良法是提取番茄叶片总RNA最适宜的方法。对番茄不同时期果实而言,华越洋试剂盒法提取青果期、转色期果实总RNA的效果最好,其浓度最高,且琼脂糖凝胶电泳条带清晰完整、无拖尾现象,但成本最高;而提取成熟期果实RNA的最优方法是Trizol改良法,完整性最好,成本较低。研究为开展后续相关基因表达及功能研究等分子生物学研究提供了技术基础。Taking tomato variety Moneymaker(MM)as the material,compared the extraction effects of three methods including kit method,Trizol method and Trizol improved method on total RNA from tomato leaves and fruits at different stages,and measured the concentration,purity and integrity of total RNA extracted samples by nucleic acid protein detector and agarose gel electrophoresis re⁃spectively.The results showed that in terms of tomato leaves,the purity of total RNA extracted by the three methods was good;Among the three methods,Trizol improved method was the most suitable method for extracting total RNA of tomato leaves.For the tomato fruits of different periods,the Huayueyang kit method had the best effect of extracting total RNA from the fruit in the green fruit stage and the color transformation stage,with the highest concentration.The cost was the highest,and the best method for extracting the ma⁃ture fruit RNA was the Trizol improved method,which had the best integrity and lower cost.Functional research and other molecular biology research provide a technical basis.

关 键 词：番茄(Solanum lycopersicum) 叶片 果实 总RNA 提取方法 

分 类 号：Q522[生物学—生物化学] S641.2[农业科学—蔬菜学]