烟草西柏三烯二醇含量的遗传分析  

Genetic Analysis of Cembratrien-diols Content in Tobacco

作　　者：鞠馥竹 张洪博[1] 闫宁[1] 杜咏梅[1] 张忠锋[1] 付秋娟[1] 柳林青 刘静 刘艳华[1] JU Fuzhu;ZHANG Hongbo;YAN Ning;DU Yongmei;ZHANG Zhongfeng;FU Qiujuan;LIU Linqing;LIU jing;LIU Yanhua(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101;Graduate School,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]中国农业科学院研究生院,北京100081

出　　处：《中国烟草科学》2021年第1期1-6,共6页Chinese Tobacco Science

基　　金：中国烟草总公司科技重大专项项目[10201501004(XX-04)];中国农业科学院科技创新工程(ASTIP-TRIC05)。

摘　　要：烟草西柏三烯二醇(CBT-diols)不仅是烟叶中香气成分的重要前体物质,还具有抗癌、抗菌等生物活性,为揭示CBT-diols含量的遗传模型及遗传效应,本研究以高CBT-diols含量种质织金黑吊把和低CBT-diols含量种质Mont Clame Brun为亲本配制杂交组合,获得P1、P2、F1、F2的四世代遗传分析群体,测定F2群体单株CBT-diols含量,利用“主基因+多基因”混合遗传模型对CBT-diols含量相关基因进行遗传分析。结果表明,α-CBT-diol、β-CBT-diol与CBT-diols含量3个性状均受2对加性-显性-上位性主基因+加性-显性-上位性多基因(E-0)控制,2对主基因的加性效应均为正值,显性效应值均为负值,且第1对主基因的加性效应及显性效应均高于第2对。CBT-diols含量3个性状的主基因遗传率分别为76.96%、76.88%、77.55%,多基因遗传率不到1%。因此,CBT-diols含量主要受2对主基因遗传效应的影响。Cembratrien-diols(CBT-diols)in tobacco is not only an important precursor substance of aroma components in tobacco leaves,but also has anti-cancer,antibacterial and other medicinal values.To reveal the genetic model and genetic effect of CBT-diols content,an F2 population was developed by crossing between the high CBT-diols content germplasm Zhijinheidiaoba(maternal parent)and low CBT-diols content germplasm Mont Clame Brun(paternal parent).Genetic analysis was carried out by using joint segregation analysis method with a mixed genetic model of major gene plus polygene.Results from genetic analysis showed thatα-CBT-diol,β-CBT-diol and CBT-diols contents were controlled by 2 pairs of additive-dominant-epigenetic major genes plus additive-dominant-epigenetic polygenes(E-0).The additive effects of the two major genes were all positive,and the dominant effects were negative,and the additive and dominant effects of the first pair of major genes were stronger than those of the second pair.The heritability of the major genes of the 3 traits of CBT-diols content was 76.96%,76.88%,and 77.55%,the polygenic inheritance rate was less than 1%.In general,the genetic effect inheritance of the 2 pairs of main genes was dominant in the three traits.Therefore,the content of CBT-diols was mainly affected by genetic effect,and the genetic effects of the major genes were the main genetic effects.

关 键 词：烟草 西柏三烯二醇 主基因+多基因 遗传分析 

分 类 号：S572[农业科学—烟草工业]