姜黄素对帕金森模型大鼠损伤神经元的修复作用及相关机制研究  

Repair effect of curcumin on injured neurons in Parkinson’s disease model rats and its related mechanism

作　　者：郭森[1] 张硕 付慧霄 刘伟[2] 李静[2] 朱江[2] 张弛 GUO Sen;ZHANG Shuo;FU Huixiao(Chengde Medical University,Chengde 067000,China)

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000 [2]承德医学院附属医院神经内科,河北承德067000

出　　处：《中风与神经疾病杂志》2021年第2期128-131,共4页Journal of Apoplexy and Nervous Diseases

基　　金：2020年度河北省“三三三人才工程”资助项目(No.A202001022)。

摘　　要：目的探究姜黄素对帕金森模型大鼠损伤神经元的修复作用及相关机制。方法选取40只SD健康雄性大鼠,10只为正常组,其余30只建立帕金森模型,分为模型组、低、高剂量组,对4组大鼠进行分别干预。对各组大鼠学习记忆能力进行检测,去各组大鼠脑组织标本进行HE染色,统计神经元大小、数量,酶联免疫吸附实验法检测IL-6、IL-1β、TNF-α水平。结果与模型组、低剂量组比较,高剂量组大鼠神经元大小、数量较高(P<0.05)。与模型组、低剂量组比较,高剂量组大鼠各时间点逃避潜伏期较短,穿越平台次数较高(P<0.05)。与模型组、低剂量组比较,高剂量组大鼠IL-6、IL-1β、TNF-α水平较低(P<0.05)。结论使用姜黄素对帕金森模型大鼠进行干预,能够改善大鼠学习记忆能力,提升大鼠脑组织神经元大小、数量,发挥神经元损伤修复作用,其功能可能与减轻脑组织炎症反应,调控细胞凋亡蛋白表达有关。Objective To explore the repair effect of curcumin on injured neurons in Parkinson’s model rats and the related mechanisms.Methods Forty SD healthy male rats were selected,10 were group,the remaining 30 were established Parkinson’s model,divided into model group,low and high dose groups,and the four groups of rats were intervened separately.The learning and memory abilities of rats in each group were tested.HE staining was performed on brain tissue specimens of rats in each group.The size and number of neurons were counted.The levels of IL-6,IL-1βand TNF-αwere detected by enzyme-linked immunosorbent assay.Results Compared with model group and low dose group,the size and number of neurons in high dose group were higher(P<0.05).Compared with model group and low dose group,rats in high dose group had shorter escape latency and higher number of times crossing the platform at each time point(P<0.05).Compared with model group and low dose group,the levels of IL-6,IL-1βand TNF-αwere lower in high dose group(P<0.05).Conclusion Intervention with curcumin in Parkinson’s model rats can improve the learning and memory ability of rats,increase the size and number of neurons in rat brain tissue,and play a role in neuronal damage repair.Its function may be related to alleviating the inflammatory response in brain tissue,regulating the expression of apoptosis protein.

关 键 词：帕金森 姜黄素 神经元损伤 炎症反应 

分 类 号：R742.5[医药卫生—神经病学与精神病学]