CO2和乙酸钠对雨生红球藻生长及其虾青素积累的影响  

Effects of CO and Sodium Acetate on the Growth and Astaxanthin Accumulation of the Green Alga Haematococcus pluvialis

作　　者：叶鑫 虞新磊 胡朝阳[1] 徐年军[1] 孙雪[1] YE Xin;YU Xinlei;HU Chaoyang;XU Nianjun;SUN Xue(School of Marine Science,Ningbo University/Key Laboratory of Marine Biotechnology of Zhejiang Province,Ningbo,Zhejiang 315211)

机构地区：[1]宁波大学海洋学院/浙江省海洋生物工程重点实验室,浙江宁波315211

出　　处：《核农学报》2021年第3期613-622,共10页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(31572638)。

摘　　要：雨生红球藻(Haematococcus pluvialis)是强抗氧化剂虾青素的天然优质来源,碳源是影响雨生红球藻虾青素产量的重要因素之一。为探究CO_(2)和乙酸钠在雨生红球藻生长和虾青素积累中的作用,本研究通过测定生化指标和荧光定量PCR的方法,比较了这2种碳源对雨生红球藻干重、虾青素含量、生长相关酶及基因转录水平等的影响。结果表明,在绿色营养阶段,高CO_(2)组培养雨生红球藻干重为对照组的1.81倍(第8天),可溶性蛋白含量和核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)活力升高,磷酸烯醇式丙酮酸羧化酶(PEPC)和苹果酸脱氢酶(MDH)活力及其编码基因转录表达上调;乙酸钠组雨生红球藻干重为对照组的1.56倍(第8天),Rubisco活力及其大亚基转录表达受抑制,PEPC和MDH活力及其转录水平升高。在厚壁孢子阶段,高CO_(2)组雨生红球藻细胞状态良好,其干重和虾青素产量分别为对照组的1.96和2.40倍(第8天),3个虾青素合成相关酶编码基因的转录水平在第3天高于对照组,Rubisco、PEPC、MDH活力较高;乙酸钠组雨生红球藻干重和虾青素产量分别为对照组的1.54和1.85倍(第8天),高光胁迫1 d后藻细胞部分变红,同时3个虾青素合成相关酶的基因表达量快速升高、Rubisco活力降低,而PEPC和MDH活力升高。综上,补充CO_(2)或乙酸钠均可显著促进雨生红球藻生长和虾青素积累,而高CO_(2)浓度培养藻的状态较好,乙酸钠则可促进虾青素的快速积累。本研究结果为今后利用雨生红球藻高效生产虾青素提供了理论参考。Haematococcus pluvialis(Chlorophyta) is the optimal organism for producing strong antioxidant astaxanthin. Carbon source is one of the most important factors affecting astaxanthin production in H. pluvialis. In order to investigate the role of CO_(2) and sodium acetate(NaAc) on the growth and astaxanthin accumulation, the effects of two carbon sources on the algal dry weight, astaxanthin content, activities of growth-related enzymes and the transcriptional levels of related genes in H. pluvialis. were compared by biochemical determination and real-time quantitative PCR methods Results showed that at green vegetative stage, the dry weight of this algae was 1.81 times in 0.16% CO_(2) group relative to that of the control group on day 8;soluble protein content and ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco) activity was significantly increased;the activities and transcriptional expression of phosphoenolpyruvate carboxylase(PEPC) and malate dehydrogenase(MDH) were up-regulated by the high CO_(2) concentration. The dry weight of NaAc group was 1.56 times of that in the control group on day 8;Rubisco activity and its large subunit mRNA content were repressed, yet the activities and their transcriptional levels of PEPC and MDH were promoted by the addition of NaAc at green vegetative stage. At red cyst stage, the alga grew well, and its biomass and astaxanthin production were 1.96 times and 2.40 times of those in the control group on day 8, respectively, the mRNA levels of three astaxanthin synthesis-related enzymes were higher than control group on day 3 by high CO_(2) concentration, and the activities of Rubisco, PEPC, MDH were higher than those in the control group. However, the biomass and astaxanthin yield in the NaAc group were 1.54-and 1.85-fold higher of the control group on day 8, respectively;some algal cells began to turn red, meanwhile, the mRNA levels of three astaxanthin synthesis-related enzymes were increased rapidly on day 1;PEPC and MDH activities were stimulated, yet Rubisco activity was

关 键 词：雨生红球藻 CO2 乙酸钠 生物量 虾青素 

分 类 号：Q945[生物学—植物学]