外源赤霉素对烤烟叶面腺毛和香气物质的影响  

Effects of Exogenous Gibberellin on Flue-cured Tobacco Leaf Trichome and Aromatic Substances

作　　者：贺凌霄 薛刚[1] 孙聚涛 张智强 丁永乐[1] 杨铁钊[1] 徐世晓[1] 程昌合[2] HE Lingxiao;XUE Gang;SUN Jutao;ZHANG Zhiqiang;DING Yongle;YANG Tiezhao;XU Shixiao;CHENG Changhe(Tobacco College,Henan Agricultural University,Zhengzhou 450002,China;Technology Center,Zhejiang China Tobacco Industry Co.,Ltd.,Hangzhou 310024,China)

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]浙江中烟工业有限责任公司技术中心,浙江杭州310024

出　　处：《河南农业科学》2021年第1期52-59,共8页Journal of Henan Agricultural Sciences

基　　金：中国烟草总公司河南省公司重大专项(2018410000270035);河南省烟草公司许昌市公司科技项目(2020411000240069);贵州省烟草公司贵阳市公司科技项目(2020-07)。

摘　　要：为增加烤后烟叶中性致香成分含量,提高烟叶品质,并探究赤霉素在烤烟西柏烷类物质合成代谢途径中的作用,以NC71、中烟100为材料,苗期茎尖喷施赤霉素,并在叶龄50、60 d分别测定叶片腺毛密度、腺毛分泌物含量等相关指标。结果表明,喷施赤霉素后,烤烟叶面腺毛密度平均提高了30.97%,腺毛分泌物含量平均提高了37.44%,烤后烟叶中性致香成分平均提高了78.38%;此外,烤烟内源赤霉素含量上升及合成途径中,正调控基因CPS、GA 20ox表达量上调,负调控基因GA2 ox表达量下调。因此,苗期茎尖喷施赤霉素能提高烟株内源赤霉素含量,增加叶面腺毛密度,提高腺毛分泌能力,增加腺毛分泌物及中性致香成分萜类降解产物的含量。In order to increase the content of the neutral aroma components of flue-cured tobacco,improve the quantity,and explore the role of gibberellin in the anabolic pathway of cembranes,trichome density and trichome exudate content were detected at 50,60 days old leaves of NC71 and Zhongyan 100,which were sprayed with gibberellin at seedling stage.The results showed that the density of trichome and the content of trichome exudate increased by 30.97%and 37.44%,respectively,and the neutral aroma components of flue-cured tobacco increased by 78.38%.Moreover,the content of endogenous gibberellin increased,and the expression levels of positive regulatory genes CPS and GA 20 ox in the gibberellin synthetic pathway were up-regulated,while the expression levels of negative regulatory genes GA 2 ox was down-regulated.In conclusion,spraying gibberellin on the stem tip can increase the density of leaf trichome,the secretion capacity of trichome,the content of trichome exudate and terpene degradation products of neutral aroma components at seedling stage.

关 键 词：烤烟 外源赤霉素 叶面腺毛 腺毛分泌物 香气物质 内源赤霉素 

分 类 号：S572[农业科学—烟草工业]