一氧化氮参与盐胁迫下长春花酚类代谢的调控研究  

Regulation of NO in the Phenolics Metabolism of Catharanthus roseus under Salt Stress

作　　者：赵晓菊 张奕婷 刘佳[2] 刘洋[2] 唐中华[2] ZHAO Xiao-Ju;ZHANG Yi-Ting;LIU Jia;LIU Yang;TANG Zhong-Hua(Bioengineering Institute,Daqing Normal University,Daqing 163712;College of Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040)

机构地区：[1]大庆师范学院生物工程学院,大庆163712 [2]东北林业大学化学化工与资源利用学院,哈尔滨150040

出　　处：《植物研究》2021年第4期633-640,共8页Bulletin of Botanical Research

基　　金：大庆师范学院科学研究基金(19ZR07)。

摘　　要：为研究外源一氧化氮(NO)调控盐胁迫下长春花中酚类化合物的响应,采用液相色谱—质谱联用(LCMS)技术靶向分析梯度浓度硝普纳(SNP)处理对盐胁迫下长春花幼苗根、茎、花、叶4个部位中酚类化合物组分及含量水平的变化。结果共鉴定出L-苯丙氨酸和18种酚类物质,C6C1类5种、C6C3类5种、C6C3C6类8种,其中原儿茶酸、绿原酸、槲皮素在长春花根、茎、花、叶4个部位中均存在;不同浓度SNP处理后长春花不同部位酚类化合物响应积累明显不同,其中C6C1和C6C3小分子酚酸类化合物主要积累在根和茎中,C3C6C3类主要富集在花和叶中;L-苯丙氨酸在茎、叶中相对含量较高,盐胁迫下茎中含量显著升高,且随外源NO浓度增大呈下降趋势。外源NO影响盐胁迫下植物器官中酚类化合物的积累和变化,其中根和茎响应敏感,从种类和相对含量的角度,茎和叶更适合检测酚类化合物。In order to uncover the response of phenolic compounds of the Catharanthus roseus under salt stress regulated by exogenous nitric oxide(NO),the ingredient and content of phenolic metabolites in roots,stems,leaves,flowers of the C.roseus were detected using Liquid Chromatography-mass spectrometry(LC-MS)technology after being treated with different concentrations of Sodium Nitroprusside(SNP)under salt stress respectively.The results showed that 5 kinds of C6C1 phenolic compounds including vanillic acid,p-Hydroxybenzoic acid,Syringic acid,Protocatechuic acids and Gallic acid,and 5 kinds of C6C3 phenolic compounds including trans-p-Hydroxycinnamic acid,Chlorogenic acid,Ferulic acid,Cinnamic acid and p-Coumaric acid and 8 kinds of C6C3C6(flavonoids)including Genistein,Petunidin,Naringenin,Quercetin-3-O-rhamnoside,Myricitrin,Quercetin,Kaempferol and Rutin and L-phenylalanine were identified respectively.Among them,quercetin,chlorogenic acid,protocatechuic acids were all in roots,stems,leaves and flowers.The responsive accumulation of the phenolic compounds in different tissues of C.roseus were significantly different under different concentrations of SNP treatment respectively.The small molecular phenolic acids including C6C1and C6C3 were mainly accumulated in roots and stems and C3C6C3 phenolic compounds were enriched mainly in surface leaves and flowers respectively.There were more L-phenylalanine in stem and leaf than in root and flower,and the relative content of L-phenylalanine in stem significantly increased under salt stress,but it showed a downward trend with the increase of NO concentration.Exogenous NO affected the accumulation and change of phenolic compounds in C.roseus under salt stress,and the roots and stems were more sensitive to the response.From the perspective of species and relative content,the stems and leaves were more suitable for the detection of phenolic compounds than others.

关 键 词：盐胁迫 一氧化氮 长春花 代谢组 酚类 

分 类 号：Q945.78[生物学—植物学]