S100A8/A9和S100A4在外阴硬化性苔藓中的表达及意义  

Expression and significance of S100A8/A9 and S100A4 in vulvar sclerosing mosses

作　　者：陈晓丹 孟斐[1] 武昕[2] CHEN Xiao-dan;MENG Fei;WU Xin(Department of Obstetrics,Central Hospital Affiliated to Shenyang Medical College,Liaoning Province,Shenyang 110024,China;Department of Gynaecology,the First Affiliated Hospital of China Medical University,Liaoning Province,Shenyang 110001,China)

机构地区：[1]沈阳医学院附属中心医院产科,辽宁沈阳110024 [2]中国医科大学附属第一医院妇科,辽宁沈阳110001

出　　处：《中国当代医药》2021年第4期119-121,125,F0003,共5页China Modern Medicine

基　　金：国家自然科学基金资助项目(30973190)。

摘　　要：目的检测外阴硬化性苔藓中S100A8/A9及S100A4的表达情况,探讨S100A8/A9、S100A4与外阴硬化性苔藓的关系。方法选取中国医科大学附属第一医院妇科门诊2008年1月~2018年1月的30例外阴硬化性苔藓患者的手术标本。另选取中国医科大学附属第一医院妇科病房2013年1月~2018年1月需行子宫脱垂等阴式手术的10例患者的外阴正常皮肤。标本采用SABC免疫组织化学方法检测,分析S100A8/A9及S100A4的表达情况。结果外阴正常皮肤中,S100A8/A9阳性细胞多位于基底细胞层,S100A8/A9阳性细胞的光密度值为0.7946±0.1369;而在外阴硬化性苔藓中,S100A8/A9阳性细胞多位于表皮近全层,S100A8/A9阳性细胞的光密度值为1.6206±0.4312。外阴硬化性苔藓标本中的S100A8/A9阳性细胞光密度值高于外阴正常皮肤标本,差异有统计学意义(P<0.05)。外阴正常皮肤中,S100A4阳性细胞多位于基底细胞层,真皮层有少量间质细胞阳性表达,S100A4阳性细胞的光密度值为0.3020±0.1266;而在外阴硬化性苔藓中,S100A4阳性细胞仍位于基底层,间质近阴性表达,S100A4阳性细胞的光密度值为0.3583±0.1575。外阴硬化性苔藓、外阴正常皮肤标本中的S100A4阳性细胞光密度值比较,差异无统计学意义(P>0.05)。结论S100A8/A9参与外阴硬化性苔藓的病变过程,S100A4可能与外阴硬化性苔藓的病变过程无明确相关性。Objective To detect the expression of S100A8/A9 and S100A4 in vulvar sclerosing mosses,and to explore the relationships between S100A8/A9,S100A4 and vulvar sclerosing mosses.Methods Thirty surgical specimens were collected from the Department of Gynecology of the First Affiliated Hospital of China Medical University from January 2008 to January 2018 in patients with vulvar sclerosing mosses.The normal vulva skin samples of 10 patients who needed vaginal surgery such as uterine prolapse from January 2013 to January 2018 were selected from the Gynecological Ward of the First Affiliated Hospital of China Medical University.The samples were detected by SABC immunohistochemistry.The expression of S100A8/A9 and S100A4 were analyzed.Results In normal skin of vulva,S100A8/A9 positive cells were mostly located in basal cell layer,and the optical density of S100A8/A9 positive cells was 0.7946±0.1369.However,in vulvar sclerosing mosses,S100A8/A9 positive cells were mostly located near the whole layer of epidermis,and the optical density of S100A8/A9 positive cells was 1.6206±0.4312.The optical density of S100A8/A9 positive cells in vulvar sclerosing mosses was higher than that in normal vulva skin,and the difference was statistically significant(P<0.05).In normal skin of vulva,S100A4 positive cells were mostly located in basal cell layer,and a small amount of stromal cells were positively expressed in dermis.The optical density of S100A4 positive cells was 0.3020±0.1266.However,in mosses sclerosing vulva,S100A4 positive cells were still located in the basal layer and were negatively expressed in the interstitium.The optical density of S100A4 positive cells was 0.3583±0.1575.There was no significant difference in the optical density of S100A4 positive cells between mosses sclerosing vulva and normal vulva skin samples(P>0.05).Conclusion S100A8/A9 take a part in the pathological process of vulvar sclerosing mosses,however S100A4 may not have definite correlation with the pathological process of vulvar sclerosing mosses.

关 键 词：S100A8/A9 S100A4 外阴硬化性苔藓 免疫组化 

分 类 号：R737.3[医药卫生—肿瘤]