X射线荧光微区分析结合电感耦合等离子体质谱法进行保健食品中元素含量的测定  

Determination of Elements in Health Food by X-Ray Fluorescence Microanalysis Combined With Inductively Coupled Plasma Mass Spectrometry

作　　者：赵婷[1,2,3] 池海涛 刘奕忍[1,2,3] 高峡 黄瞾[1] 张梅 李琴梅[1,2,3] ZHAO Ting;CHI Hai-tao;LIU Yi-ren;GAO Xia;HUANG Zhao;ZHANG Mei;LI Qin-mei(Beijing Centre for Physical and Chemical Analysis,Beijing 100089,China;Beijing Key Laboratory of Organic Materials Testing Technology&Quality Evaluation,Beijing 100089,China;Beijing Engineering Research Center of Food Safety Analysis,Beijing 100089,China)

机构地区：[1]北京市理化分析测试中心,北京100089 [2]有机材料检测技术与质量评价北京市重点实验室,北京100089 [3]北京市食品安全分析测试工程技术研究中心,北京100089

出　　处：《光谱学与光谱分析》2021年第3期750-754,共5页Spectroscopy and Spectral Analysis

基　　金：北京市科学技术研究院创新团队项目(HIT201902);北京市科学技术研究院北科萌芽计划项目(BGS201904);北京市市级财政项目(PXM2020-178305-000007)资助。

摘　　要：X射线荧光光谱微区分析法既有X射线荧光光谱法快速、简便、无损检测等特点,又可对保健食品表面的元素分布进行检测,电感耦合等离子体质谱法具有检出限低、线性范围宽、多元素同时测定等优点,旨在建立一种X射线荧光光谱微区分析法和电感耦合等离子体质谱法联合测定保健食品中元素种类、分布及含量的方法。利用X射线荧光光谱微区分析技术对一种保健食品进行了元素种类及元素在样品表面的分析,并对元素含量进行了半定量分析,确定保健品中含有钙(Ca)、铁(Fe)、钌(Ru)、钼(Mo)元素。利用微波消解-电感耦合等离子体质谱法对其中含有的主要元素Ca和Fe进行了定量分析。微波消解-电感耦合等离子体质谱法测定结果Ca元素平均值为6.23%,相对标准偏差1.78%;Fe元素平均值3.82%,相对标准偏差2.14%。与X射线荧光光谱微区分析法半定量测试结果的样品中含有的Ca元素含量在6.0%~10.0%之间,样品中含有的Fe元素含量在2.0%~4.0%之间的结论一致。且通过X射线荧光光谱微区分析法得知保健食品表面的各元素分布不均匀。结果表明:X射线荧光光谱微区分析方法既可利用微量样品快速无损测得保健食品中的元素半定量含量,又可测得保健食品中的元素分布情况。结合电感耦合等离子体质谱法,还可以对样品中感兴趣的元素含量进一步检测,得到定量分析结果。X-ray fluorescence spectrum micro-area analysis method has the characteristics of rapid,simple and non-destructive testing,and can detect the distribution of elements on the surface of health food.Inductively coupled plasma mass spectrometry has the advantages of low detection limit,wide linear range and multi-element simultaneous determination.Methods:A health food was analyzed by X-ray fluorescence spectrum micro-region analysis technique,and the elemental content was semi-quantitatively analyzed to determine the elements of calcium(Ca),iron(Fe),ruthenium(Ru)and molybdenum(Mo)in health products.The main elements of Ca and Fe were quantitatively analyzed by microwave digestion-inductively coupled plasma mass spectrometry(ICP-MS).The average value of Ca element was 6.23%,the relative standard deviation was 1.78%,the average value of the Fe element was 3.82%,and the relative standard deviation was 2.14%.The results were consistent with the conclusion that the content of Ca in the sample with X-ray fluorescence spectrum micro-analysis was between 6.0% and 10.0%,and the content of Fe in the sample was between 2.0% and 4.0%.The distribution of the elements on the surface of health food was not uniform by X-ray fluorescence spectrum analysis.The X-ray fluorescence spectrum micro-area analysis method cannot only measure the elemental semi-quantitative content in health food by using the trace sample,but also measure the elemental distribution in health food.Combined with inductively coupled plasma mass spectrometry,the content of the elements of interest in the sample can be further detected,and the quantitative analysis results can be obtained.

关 键 词：X射线荧光光谱法(XRF) X射线荧光光谱微区 电感耦合等离子体质谱法(ICP-MS) 保健食品 

分 类 号：O657.3[理学—分析化学]