干扰NFAT5的表达对宫颈癌增殖和转移能力的影响及机制研究  

Effects and Mechanism of Interfering by the Expression of NFAT5 on the Proliferation and Metastasis of Cervical Cancer

作　　者：李剑锋 倪莎[2] 刘笑默 LI Jianfeng;NI Sha;LIU Xiaomo(Department of Obstetrics and Gynecology,Maternity and Children′s Hospital of Yingkou City,Yingkou 115000,China;Department of Obstetrics and Gynecology,Shengjing Hospital Affiliated to China Medical University,Shenyang 110003,China)

机构地区：[1]辽宁省营口市妇产儿童医院妇产科,辽宁营口115000 [2]中国医科大学附属盛京医院妇产科,辽宁沈阳110003

出　　处：《标记免疫分析与临床》2021年第1期117-121,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的研究活化T细胞的核因子(NFAT5)对宫颈癌细胞增殖和转移能力的影响,并探讨其可能的作用机制。方法检测NFAT5在宫颈癌细胞系C33A、HeLa、Caski和人宫颈上皮永生化细胞H8中的表达,选择表达最高的宫颈癌细胞系进行NFAT5 siRNA感染,分为对照组(si-NC组)和实验组(si-NFAT5组)。Western blotting检测各组细胞中NFAT5的表达水平;MTS、平板克隆和EdU实验检测各组细胞的增殖能力;Transwell和划痕实验检测各组细胞转移能力;Western blotting检测NFAT5对Toll样受体-4(TLR4)/髓样分化因子88(MyD88)信号通路活化的影响。结果Western blotting检测结果显示NFAT5在宫颈癌细胞系的表达水平高于在人宫颈上皮永生化细胞H8中的表达(P<0.05),选择表达水平最高的HeLa细胞转染NFAT5 siRNA进行后续实验。Western blotting结果显示NFAT5在si-NFAT5组中的表达降低(P<0.05);si-NFAT5组HeLa细胞增殖和转移能力下降,细胞中TLR4和MyD88蛋白的表达降低。结论干扰NFAT5的表达可能通过降低TLR4/MyD88信号通路活性抑制宫颈癌细胞增殖和转移能力,NFAT5可能是宫颈癌治疗的新型靶标。Objective To investigate the effects of nuclear factor of activated T cells(NFAT5)on the proliferation and metastasis of cervical cancer cells,and to explore its possible mechanism of action.Methods The expressions of NFAT5 in cervical cancer cell lines C33A,HeLa,Caski and human cervical epithelial cells H8 were detected,and the highest expression cervical cancer cell line was selected for NFAT5 siRNA infection,which was divided into the control group(si-NC group)and experimental group(si-NFAT5 group).Western blotting was used to detect the expression of NFAT5 of cells in each group.MTS,plate clone and EdU were used to detect the proliferative capacity of cells in each group.Transwell and wound scratch assay were used to detect the cell metastasis ability of each group.Western blotting was used to detect the activation of TLR4/MyD88 signaling pathway affected by NFAT5.Results Western blotting test results showed that the expression level of NFAT5 in cervical cancer cell lines was higher than that in human cervical epithelial cells H8(P<0.05),and the highest expression of HeLa cells was transfected with NFAT5 siRNA for subsequent experiments.Western blotting test results also showed that the expression of NFAT5 was decreased in the si-NFAT5 group(P<0.05).The proliferation and metastasis ability of HeLa cells in the si-NFAT5 group were decreased,and the expressions of TLR4 and MyD88 proteins were decreased.Conclusion Interfering with the expression of NFAT5 may inhibit the proliferation and metastasis of cervical cancer cells by decreasing the activity of TLR4/MyD88 signaling pathway.NFAT5 could be a novel target for the treatment of cervical cancer.

关 键 词：宫颈癌 活化T细胞的核因子 增殖 侵袭 TLR4/MyD88 

分 类 号：R737.33[医药卫生—肿瘤]