胰腺癌组织中转化生长因子β1、上皮间质转化相关蛋白表达水平及其临床意义  

Expression of transforming growth factor-β1 and epithelial mesenchymal transition related proteins in pancreatic cancer tissue and its clinical significance

作　　者：施景龙 李博[2] 贺欢[3] 宋向晖[1] SHI Jing-long;LI Bo;HE Huan;SONG Xiang-hui(Department of General Surgery,Guangzhou Twelfth People's Hospital,Guangdong Province,Guangzhou 510630,China;Department of Gastroenterology,the Second People's Hospital of Guangdong Province,Guangzhou 510317,China;ICU,Guangdong Maternity and Child Health Hospital,Guangzhou 510000,China)

机构地区：[1]广州市第十二人民医院普通外科,广东广州510630 [2]广东省第二人民医院消化内科,广东广州510317 [3]广东省妇幼保健院ICU,广东广州510000

出　　处：《中国当代医药》2021年第5期4-8,共5页China Modern Medicine

基　　金：广东省医学科学技术研究基金项目(A2019279)。

摘　　要：目的探讨胰腺癌组织中转化生长因子β1(TGF-β1)、上皮间质转化(EMT)相关蛋白表达水平及其临床意义。方法选取2019年1月~2020年2月广州市第十二人民医院普通外科和广东省第二人民医院消化内科收治的60例胰腺癌患者作为研究对象。免疫组织化学染色法检测其胰腺癌组织和癌旁组织的TGF-β1以及E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)EMT相关蛋白阳性表达率。检测患者血清糖类抗原(CA)199和CA242水平。比较不同胰腺癌组织TGF-β1和EMT相关蛋白表达情况患者的血清CA199和CA242水平、淋巴结转移、浸润分期状况,并分析胰腺癌组织TGF-β1和EMT相关蛋白表达与其血清CA199和CA242水平、淋巴结转移、浸润分期的关系。结果胰腺癌组织中E-cadherin的阳性表达率低于癌旁组织,而TGF-β1和Vimentin阳性表达率则于高癌旁组织,差异有统计学意义(P<0.05)。E-cadherin阳性表达患者的血清CA199和CA242水平、淋巴结转移率、浸润分期为T_(3)~T_(4)期比例均低于其阴性表达患者,差异有统计学意义(P<0.05);TGF-β1阳性表达患者的血清CA199和CA242水平、淋巴结转移率、浸润分期为T_(3)~T_(4)期比例均高于其阴性表达患者,差异有统计学意义(P<0.05);Vimentin阳性表达患者的血清CA199和CA242水平、淋巴结转移率、浸润分期为T_(3)~T_(4)期比例亦均高于其阴性表达患者,差异有统计学意义(P<0.05)。Kendall相关分析结果显示,胰腺癌组织TGF-β1和Vimentin阳性表达率与其血清CA199和CA242水平、淋巴结转移率、浸润分期均呈正相关(P<0.05),而胰腺癌组织E-cadherin阳性表达率与其血清CA199和CA242水平、淋巴结转移率、浸润分期均呈负相关(P<0.05)。结论胰腺癌组织TGF-β1和Vimentin表达上调而E-cadherin表达下调且均与肿瘤侵袭转移相关,可能用于胰腺癌侵袭转移评估。Objective To investigate the expression of transforming growth factor-β1(TGF-β1)and epithelial mesenchymal transition(EMT)related proteins in pancreatic cancer tissue and its clinical significance.Methods A total of 60 patients with pancreatic cancer admitted to the Department of General Surgery of Guangzhou Twelfth People's Hospital and Gastroenterology Department of the Second People's Hospital of Guangdong Province from January 2019 to February 2020 were selected as research objects.The positive expression rates of TGF-β1 and EMT related proteins such as E-cadherin and Vimentin of pancreatic cancer tissue and paracancerous tissue of the patients were detected by immunohistochemistry method.The levels of serum carbohydrate antigen(CA)199 and CA242 were measured.The levels of serum CA199 and CA242,lymph node metastasis and infiltration stage in patients with different expression levels of TGF-β1 and EMT related proteins in pancreatic cancer tissues were compared.And the relationship between the expression of TGF-β1 and EMT related proteins with the levels of serum CA199 and CA242,lymph node metastasis and infiltration stage in pancreatic cancer was analyzed.Results The positive expression rate of E-cadherin in pancreatic cancer tissue was lower than that in paracancerous tissue,while the positive expression rates of TGF-β1 and Vimentin in pancreatic cancer tissue were higher than those in paracancerous tissue,and the differences were statistically significant(P<0.05).The levels of serum CA199 and CA242,the rate of lymph node metastasis and the proportion of infiltration stage as T_(3)-T_(4) of patients with E-cadherin positive expression in pancreatic cancer tissue were lower than those of the patients with E-cadherin negative expression in pancreatic cancer tissue,and the differences were statistically significant(P<0.05).The levels of serum CA199 and CA242,the rate of lymph node metastasis and the proportion of infiltration stage as T_(3)-T_(4) of patients with TGF-β1 positive expression in pancreatic c

关 键 词：胰腺癌 癌组织 转化生长因子Β1 上皮间质转化相关蛋白 表达 侵袭转移 

分 类 号：R735[医药卫生—肿瘤]