AhBG1 转基因拟南芥的ABA敏感性和抗旱性研究  

The ABA Sensitivity and Drought Resistance of AhBG1 Transgenic Arabidopsis

作　　者：黄莹琳 张志 张洪亮 胡博[1,2] 龙海涛 HUANG Yinglin;ZHANG Zhi;ZHANG Hongliang;HU Bo;LONG Haitao(School of Life Sciences,South China Normal University∥Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,Guangzhou 510631;SCNU Qingyuan Institute of Science and Technology Innovation Co.,Ltd.,Qingyuan 511517)

机构地区：[1]华南师范大学生命科学学院∥广东省植物发育生物工程重点实验室,广州510631 [2]华南师大(清远)科技创新研究院有限公司,清远511517

出　　处：《华南师范大学学报（自然科学版）》2021年第1期56-62,共7页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31471422);广东省科技创新战略专项资金项目(2018A030313629)。

摘　　要：为探究花生基因AhBG1对拟南芥ABA敏感性和抗旱性的影响,以过表达AhBG1拟南芥为材料,检测其ABA敏感性及脱水处理下ABA质量分数、叶片失水率、干旱存活率及ABA稳态相关基因表达变化.结果表明:AhBG1是编码花生β-葡萄糖苷酶的家族成员,定位于细胞质;与野生型相比,AhBG1过表达拟南芥植株在干旱条件下体内ABA水平提高,干旱存活率增加,增强有关生物合成途径和信号转导途径相关基因上调,抑制氧化代谢途径相关基因表达.AhBG1蛋白可能催化ABA-GE形成ABA,提高植物体内ABA的质量分数,通过影响ABA稳态相关基因的表达从而提高植物的抗旱性.To explore the effects of peanut gene AhBG1 on ABA sensitivity and drought resistance of Arabidopsis thaliana,the ABA sensitivity,ABA content,water loss rate,survival rate and expression levels of genes related to ABA homeostasis of AhBG1 transgenic Arabidopsis were detected under drought stress.The results showed that AhBG1 is a member of peanutβ-glucosidase family,which is located in the cytoplasm.Compared with the wild type,the AhBG1 overexpression in Arabidopsis thaliana increased ABA level and drought survival rate under drought stress,enhanced expression of genes related to biosynthetic pathway and signal transduction pathway,and inhibited the expression of genes related to oxidative metabolic pathway.AhBG1 may catalyze ABA-GE to form ABA and thus increase ABA content in plants and influence the expression of ABA homeostasis-related genes to improve drought tolerance of plants.

关 键 词：AhBG1 Β-葡萄糖苷酶 ABA敏感性 抗旱性 

分 类 号：Q786[生物学—分子生物学]