我国四株埃可病毒6型全基因组序列及重组特征分析  

Complete Genome Sequence Characteristics and Recombination Analysis of Four Echovirus 6 Strains Isolated in China

作　　者：程文隽 蔡茹[1] 冀天娇[2] 陈雨[3] 马江涛[4] 单军 于秋丽 张勇[2] 严冬梅[2] 杨倩[2] 宋洋[2] 路环环 张珂艺 许文波[2] CHENG Wenjun;CAI Ru;JI Tianjiao;CHEN Yu;MA Jiangtao;SHAN Jun;YU Qiuli;ZHANG Yong;YAN Dongmei;YANG Qian;SONG Yang;LU Huanhuan;ZHANG Keyi;XU Wenbo(Medical School,Anhui University of Science and Technology,Huainan 232001,China;WHO WPRO Regional Polio Reference Laboratory,National Health Commission Key Laboratory for Medical Virology,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Hunan Center for Disease Control and Prevention,Changsha 410005,China;Ningxia Center for Disease Control and Prevention y Yinchuan 750004,China;Jiangsu Center for Disease Control and Prevention,Nanjing 210000,China;Hebei Center for Disease Control and Prevention,Shijiazhuang 050000,China)

机构地区：[1]安徽理工大学医学院,淮南232001 [2]中国疾病预防控制中心病毒病预防控制所脊髓灰质炎室,世界卫生组织西太平洋区脊髓灰质炎参比实验室,国家卫生健康委员会医学病毒与病毒病重点实验室,北京102206 [3]湖南省疾病预防控制中心,长沙410005 [4]宁夏疾病预防控制中心,银川750004 [5]江苏疾病预防控制中心,南京210000 [6]河北疾病预防控制中心,石家庄050000

出　　处：《病毒学报》2021年第1期123-132,共10页Chinese Journal of Virology

基　　金：“十三五”科技重大专项(项目号:2018ZX10201002-003-003),题目:手足口病流行特征及变异变迁规律的研究;“十三五”科技重大专项(项目号:2018ZX10713002),题目:病毒性传染病病原谱和病毒基因变异变迁规律研究。

摘　　要：埃可病毒6型(Echovirus 6,ECHO6)作为肠道病毒B组(Enterovirus B,EV-B)的一员,在临床上常导致无菌性脑膜炎(Aseptic meningitis,AM)、急性弛缓性麻痹(Acute flaccid paralysis,AFP)、手足口病(Hand,foot,and mouth disease,HFMD)等多种疾病。本文挑选四株中国大陆地区不同年份、地区及疾病严重程度的代表毒株进行全基因组测定分析,与GenBank上ECHO6全长代表株及EV-B组原型株比对分析,了解其进化特征,并筛选BLAST结果的EV-B组流行株,探究我国流行的ECHO6与其他血清型的基因重组特征。结果提示,本研究四株ECHO6毒株在非结构蛋白区与EV-B其他血清型均发生重组,其中死亡病例标本重组模式更为复杂,可能与疾病严重程度有关。通过本研究分析,可进一步揭示ECHO6的遗传特征,为肠道病毒血清型研究提供基础数据,对了解其进化变异及临床致病力影响具有重要意义。Echovirus 6(ECHO6),a member of enterovirus B(EV-B),often causes aseptic meningitis(AM),acute flaccid paralysis(AFP),hand,foot,and mouth disease(HFMD)and many other diseases.To understand their evolutionary characteristics,we analyzed four representative strains selected by different years,regions and disease severity in China’s mainland,and compared them with the full-length representative strains of ECHO6 downloaded from GenBank and the prototype strains of the EV-B group.Meanwhile,we filtered the epidemic strains of the EV-B group from the BLAST results to explore the genetic recombination characteristics of ECHO6 and other serotypes in China.Results showed that the four ECHO6 strains have recombined in the non-structural protein region with other EV-B serotypes.Among them,the recombination pattern in specimens of death cases is more complicated,which suggests relevance with the severity of the disease.This study can further reveal the genetic characteristics of ECHO6,and provide basic data for study of enterovirus,and with great significance for evolutionary variation and clinical pathogenicity.

关 键 词：埃可病毒6型(ECHO6) 全基因组序列 基因重组 

分 类 号：R373.2[医药卫生—病原生物学]