Mitochondrial transfer from bone-marrow-derived mesenchymal stromal cells to chondrocytes protects against cartilage degenerative mitochondrial dysfunction in rats chondrocytes  

作　　者：Rui Wang Talatibaike Maimaitijuma Yuan-Yuan Ma Yang Jiao Yong-Ping Cao 

机构地区：[1]Department of Orthopaedics,Peking University First Hospital,Beijing 100034,China [2]AnimaI Experiment Center,Peking University First Hospital,Beijing 100034,China

出　　处：《Chinese Medical Journal》2021年第2期212-218,共7页中华医学杂志（英文版）

基　　金：supported by a grant from the Scientific Research Seed Fund of Peking University First Hospital(No.2018SF020)。

摘　　要：Background:Previous studies have reported that mitochondrial dysfunction participates in the pathological process of osteoarthritis(OA).However,studies that improve mitochondrial function are rare in OA.Mitochondrial transfer from mesenchymal stem cells(MSCs)to OA chondrocytes might be a cell-based therapy for the improvement of mitochondrial function to prevent cartilage degeneration.This study aimed to determine whether MSCs can donate mitochondria and protect the mitochondrial function and therefore reduce cartilage degeneration.Methods:Bone-marrow-derived mesenchymal stromal cells(BM-MSCs)were harvested from the marrow cavities of femurs and tibia in young rats.OA chondrocytes were gathered from the femoral and tibial plateau in old OA model rats.BM-MSCs and OA chondrocytes were co-cultured and mitochondrial transfer from BM-MSCs to chondrocytes was identified.Chondrocytes with mitochondria transferred from BM-MSCs were selected by fluorescence-activated cell sorting.Mitochondrial function of these cells,including mitochondrial membrane potential(Δψm),the activity of mitochondrial respiratory chain(MRC)enzymes,and adenosine triphosphate(ATP)content were quantified and compared to OA chondrocytes without mitochondrial transfer.Chondrocytes proliferation,apoptosis,and secretion ability were also analyzed between the two groups.Results:Mitochondrial transfer was found from BM-MSCs to OA chondrocytes.Chondrocytes with mitochondrial from MSCs(MSCs+OA group)showed increased mitochondrial membrane potential compared with OA chondrocytes without mitochondria transfer(OA group)(1.79±0.19 vs.0.71±0.12,t=10.42,P<0.0001).The activity of MRC enzymes,including MRC complex I,II,III,and citrate synthase was also improved(P<0.05).The content of ATP in MSCs+OA group was significantly higher than that in OA group(161.90±13.49 vs.87.62±11.07 nmol/mg,t=8.515,P<0.0001).Meanwhile,we observed decreased cell apoptosis(7.09%±0.68%vs.15.89%±1.30%,t=13.39,P<0.0001)and increased relative secretion of type II collagen(2.01±0.14 v

关 键 词：Mitochondrial transfer Osteoarthritis Mitochondrial dysfunction CHONDROCYTE BM-MSCs 

分 类 号：R684.3[医药卫生—骨科学]