B7家族负性共刺激分子在急性髓系白血病中的表达特征  

The expression characteristic of inhibitory B7 family molecules in acute myeloid leukemia

作　　者：张凌逸 张巍[3] 林江[2] 陈巧云[2] 钱军[3] 袁倩 周静东[3] 张婷娟 周红[1] ZHANG Lingyi;ZHANG Wei;LIN Jiang;CHEN Qiaoyun;QIAN Jun;YUAN Qian;ZHOU Jingdong;ZHANG Tingjuan;ZHOU Hong(School of Medicine,Jiangsu University,Jiangsu Zhenjiang 212013,China;Laboratory Center,the Affiliated People's Hospital of Jiangsu University,Jiangsu Zhenjiang 212002,China;Department of Hematology,the Affiliated People's Hospital of Jiangsu University,Jiangsu Zhenjiang 212002,China)

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]江苏大学附属人民医院中心实验室,江苏镇江212002 [3]江苏大学附属人民医院血液内科,江苏镇江212002

出　　处：《现代肿瘤医学》2021年第5期872-877,共6页Journal of Modern Oncology

基　　金：江苏省基础研究计划青年基金资助项目(编号:BK20180280);镇江市社会发展项目(编号:SH2016045);江苏大学附属人民医院博士启动基金资助项目(编号:KFB201603)。

摘　　要：目的:探讨B7家族4种负性共刺激分子B7-H1、B7-H3、B7-H4和B7-H6在人急性髓系白血病(AML)中的表达及分布特征。方法:采用流式细胞术检测8株AML细胞株和22例初治AML患者骨髓原始细胞中B7-H1、B7-H3、B7-H4和B7-H6膜蛋白的表达,设8例志愿者骨髓血细胞为对照,并应用阳性表达率、平均荧光强度(MFI)及原始细胞和淋巴细胞的MFI比值(MFI比)分析各B7分子的表达特征。结果:AML细胞株中,多数细胞膜表面表达B7-H3和B7-H6,B7-H3表达较强的细胞株有SKM-1和SHI-1,B7-H6表达较强的细胞株有K562和HL-60;B7-H1仅在HEL中表达较强,而其余AML细胞中基本不表达;B7-H4在AML细胞株中均不表达。初治AML患者的骨髓血细胞中,B7-H3和B7-H6在原始细胞膜表面的表达率及原始细胞和淋巴细胞的MFI比均显著高于对照组;B7-H3高表达组的外周血白细胞数显著高于低表达组,B7-H6高表达组的年龄也显著高于其低表达组;B7-H3和B7-H6在急性单核细胞白血病患者中表达显著升高。B7-H1和B7-H4在初治AML患者中的表达水平低下或不表达,且与对照组相比均无显著差异。结论:4种B7家族负性共刺激分子在AML中的表达和分布存在差异。B7-H3和B7-H6在初治AML患者的原始细胞中异常高表达,提示可能参与急性髓系白血病的发生发展。Objective:To investigate the expression and distribution of B7-H1,B7-H3,B7-H4 and B7-H6 in human acute myeloid leukemia(AML).Methods:The membrane protein expression of B7-H1,B7-H3,B7-H4 and B7-H6 in 8 AML cell lines and bone marrow blast cells of 22 de novo AML patients were detected by flow cytometry,with that of 8 volunteers as control.The expression rate of positive cells,mean fluorescence intensity(MFI) and mean fluorescence intensity ratio of blast cells and lymphocytes(MFI ratio) were used to evaluate the expression characteristic of these B7 molecules.Results:Majority of AML cell lines expressed high or detectable level of membrane protein B7-H3 and B7-H6.The expression of B7-H3 was higher in SKM-1 and SHI-1,and B7-H6 had higher expression in K562 and HL-60.B7-H1 was highly expressed only in HEL,while B7-H1 membrane protein was not expressed in other AML cell lines.B7-H4 was undetectable in AML cell lines.The expression rate and MFI ratio of membrane protein B7-H3 and B7-H6 in de novo AML blast cells was significantly higher than that of control.And peripheral white blood cells of group with high B7-H3 expression were significantly higher when compared with group with low B7-H3 expression,and age of the group with high B7-H6 expression was also significantly higher than that of the group with low B7-H6 expression.Expression of B7-H3 and B7-H6 was elevated in patients with acute monocytic leukemia.The expression of B7-H1 and B7-H4 in de novo AML patients was low or undetectable,and there was no significant difference compared with control.Conclusion:There are differences among expression characteristics of four inhibitory B7 family molecules in AML cell lines and patients.The abnormally elevated expression of B7-H3 and B7-H6 in de novo AML suggests that the two B7 family molecules may be involved in occurrence and progression of acute myeloid leukemia.

关 键 词：B7共刺激分子 流式细胞术 急性髓系白血病 膜蛋白 表达 

分 类 号：R733.71[医药卫生—肿瘤]