黄连素对脊髓损伤大鼠染色病理、骨形态发生蛋白表达及神经元活性的影响  

Study of BBR on HE Pathology,BMP-4 mRNA Expression and Neuronal Activity in Rats with Spinal Cord Injury

作　　者：吴建军 梁珪清 郑昌坤 徐强 徐佳隆 WU Jianjun;LIANG Guiqing;ZHENG Changkun;XU Qiang;XU Jialong(Fuzhou Second Hospital Affiliated of Xiamen University,Fuzhou 350007,China;Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]厦门大学附属福州第二医院,福州350007 [2]福建中医药大学

出　　处：《中国中医骨伤科杂志》2021年第2期5-9,14,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：福建省自然科学基金项目(2018J01364)。

摘　　要：目的:研究黄连素对脊髓损伤大鼠苏木精-伊红(HE)病理、骨形态发生蛋白4(BMP-4)mRNA表达及神经元活性的影响。方法:将广东省医学SD大鼠中心的大鼠随机分为脊髓损伤组(S组)、健康对照组(H组)、黄连素组(B组),每组6只。采用流式细胞法、TUNEL法、HE染色法、免疫组化法、qRT-PCR法检测S组、B组、H组大鼠脊髓细胞凋亡数量、脊髓细胞病变程度、脊髓细胞中BMP-4蛋白阳性表达、脊髓细胞中BMP-4 mRNA的表达量、脊髓细胞凋亡率的差异性来探究黄连素对脊髓损伤大鼠HE病理、BMP-4 mRNA表达及神经元活性的影响。结果:S组脊髓细胞凋亡数量显著多于B组(P<0.05),与H组相比较,B组脊髓细胞凋亡数量显著多于H组,差异有统计学意义(P<0.05)。B组神经元细胞的凋亡情况与S组相比较显著减少,差异有统计学意义(P<0.05)。与S组比较,B组大鼠脊髓水肿减轻,损伤程度有好转趋势,细胞坏死数目有所减少。与S组相比较,B组脊髓细胞中BMP-4阳性数显著降低,差异有统计学意义(P<0.05)。与B组相比较,H组细胞BMP-4阳性数显著下降,差异有统计学意义(P<0.05);与S组相比较,B组大鼠BMP-4 mRNA表达量显著降低,BMP-2 mRNA表达量显著上升,差异有统计学意义(P<0.05);与B组相比较,H组BMP-4 mRNA表达量显著降低,BMP-2 mRNA表达量显著上升,差异有统计学意义(P<0.05)。结论:黄连素对脊髓损伤大鼠具有一定治疗作用,黄连素干预后能够显著减少大鼠脊髓细胞的凋亡数量,降低凋亡率,同时改善大鼠脊髓细胞的病理形态,促进康复,黄连素对脊髓损伤大鼠的作用机制可能与下调脊髓细胞中BMP-4的表达有关。Objective:To study the effects of berberine(BBR)on hematoxylin eosin(HE)pathology,bone morphogenetic protein 4(BMP-4)mRNA expression and neuronal activity in rats with spinal cord injury.Methods:The rats in the Guangdong medical SD rat center were randomly divided into spinal cord injury group(S group);healthy control group(H group);berberine group(B group),each with 6 rats.Flow cytometry,TUNEL method,HE staining method,immunohistochemistry method,and qRT-PCR method were used to detect the number of spinal cord cell apoptosis,the degree of spinal cord cytopathy,the expression BMP-4 protein and mRNA in spinal cord cells and the difference in the apoptosis rate of spinal cord cells in groups S,B,and H to explore the effects of berberine(BBR)on HE pathology,BMP-4 mRNA expression and neuronal activity in rats with spinal cord injury.Results:The number of apoptotic cells in B group was significantly more than that in S group(P<0.05).The apoptosis of neurons in B group was significant less than that in H group(P<0.05).Compared with S group,the spinal cord edema in B group was alleviated,the degree of injury tended to improve,and the number of cell necrosis decreased.Compared with S group,the number of BMP-4 positive cells in B group was significantly lower than S group(P<0.05).Compared with B group,the number of BMP-4 positive cells in H group was significantly decreased(P<0.05).Compared with S group,the expression of BMP-4 mRNA and BMP-2 mRNA in B group were significantly lower than S group(P<0.05).Compared with B group,the expression of BMP-4 mRNA and BMP-2 mRNA in H group were significantly decreased and significantly increased(P<0.05).Conclusion:Berberine(BBR)has a certain therapeutic efficacy for spinal cord injury of rats.The number of rat spinal cord apoptosis can be significantly reduced after BBR intervention,and the rate of apoptosis is reduced.At the same time,berberine(BBR)can improve the pathological morphology of rat spinal cord cells and promote recovery.The mechanism of BBR on spinal cord injury rats ma

关 键 词：黄连素 神经元活性 骨形态发生蛋白4(BMP-4) 脊髓损伤 

分 类 号：R-33[医药卫生]