CENPF在有丝分裂及肿瘤发生中的研究进展  

Role of CENPF in Mitosis and Tumorigenesis

作　　者：周成杰[1] 韩哲 王星月 梁成光[1] ZHOU Chengjie;HAN Zhe;WANG Xingyue;LIANG Chengguang(State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock,School of Life Science,Inner Mongolia University,Hohhot 010070,China)

机构地区：[1]内蒙古大学生命科学学院省部共建草原家畜生殖调控与繁育国家重点实验室,呼和浩特010070

出　　处：《内蒙古大学学报（自然科学版）》2021年第1期104-112,共9页Journal of Inner Mongolia University：Natural Science Edition

基　　金：内蒙古自治区自然科学基金项目(2019ZD09,2019BS03027);内蒙古自治区关键技术攻关计划项目(2019GG103);内蒙古自治区科技重大专项项目(SKL-IT-201807,2020ZD0008);内蒙古自治区高等学校科学研究项目(NJZY20007)。

摘　　要：对于有丝分裂和减数分裂而言,着丝粒是不可或缺的重要功能元件。近几年的研究表明,着丝粒蛋白F(Centromere Protein F,CENPF)是细胞周期调控的关键蛋白。CENPF是一种分子量为367kDa的核定位蛋白,它在有丝分裂前期开始增加,在有丝分裂期定位于动粒,末期开始迅速降解。CENPF缺失会造成有丝分裂缺陷,包括纺锤体无法正常组装、染色体非正常排列和分离,甚至细胞死亡。同时,CENPF可能与纺锤体组装检验点(Spindle Assembly Checkpoint,SAC)的活性有关。CENPF功能的发挥取决于法尼基化修饰,CENPF的过表达则可能导致肿瘤的发生,通过法尼基转移酶抑制剂(Farnesyltransferase Inhibitor,FTI)处理细胞则会抑制肿瘤细胞的增殖。同时,CENPF对小鼠早期胚胎的发育也具有重要的调控作用。对近些年CENPF的研究进展进行了综述,并提出了尚待解决的关键问题,希望可以为后续的研究提供一些思路。Centromere is an indispensable functional element for mitosis and meiosis.Recent studies showed that centromere protein F(CENPF)is a key regulator in cell cycle.CENPF is a nuclear protein with molecular weight of 367 kDa.Its expression level increase at prophase,then it locates in the kinetochores during metaphase and proteolyse rapidly at the onset of anaphase.Silencing of CENPF induces mitotic defects,including incorrect spindle assembly,misaligned chromosomes,abnormal chromosome segregation,even cell death.CENPF is also associated with the activity of spindle assembly checkpoint(SAC).CENPF function depends on its farnesylation.Overexpression of CENPF may lead to tumorigenesis,and farnesyltransferase inhibitor(FTI)can inhibit proliferation of tumor cells.Moreover,CENPF is an important regulator during mouse embryonic development.The research progress of CENPF was reviewed and new questions about CENPF were raised,this may provide directions for further studies.

关 键 词：CENPF 有丝分裂 法尼基化 肿瘤发生 

分 类 号：Q291[生物学—细胞生物学]