3-氰基吡啶转烟酸菌株的筛选及发酵条件研究  

Screening and Fermentation Conditions of 3-Cyanopyridine to Nicotinic Acid

作　　者：金如昌 杨竞成 李玉山 夏伟 JIN Ru-chang;YANG Jing-cheng;LI Yu-shan;XIA Wei(Anhui Redpont Biotechnology Co.Ltd.,Maanshan 243100,China)

机构地区：[1]安徽瑞邦生物科技有限公司,安徽马鞍山243100

出　　处：《安徽化工》2021年第1期55-57,共3页Anhui Chemical Industry

摘　　要：从土壤中筛选出高效催化3-氰基吡啶转化烟酸的菌株NA-6,对该菌株进行形态鉴定,初步鉴定为恶臭假单胞菌。同时对发酵条件进行研究,结果表明:该菌株在温度30℃,起始pH值为7.0,3-氰基吡啶浓度为0.3%时,腈水解酶活性最大,比原始提高了1.89倍。Nicotinic acid was produced through direct hydrolysis of 3-cyanopyridine by NA-6.The strain isolated from soil samples NA-6 was identified as pseudomonas putida by morphological identification.The results showed that optimum reac⁃tion temperature,pH,3-cyanopyridine concentration were 30℃,7.0,0.3%.The activity of nitrilase was the highest and increasing about 1.89 times than original.

关 键 词：烟酸 3-氰基吡啶 腈水解酶 筛选 发酵 

分 类 号：Q93[生物学—微生物学]