一株分离自口腔的鼠李糖乳杆菌及其特性  

In vitro assessment of probiotic properties of Lactobacillus rhamnosus isolated from oral cavity

作　　者：鄢梦洁 路江浩 孙新凯 霍文敏 马新颖 崔文静 何方[2] 赵林森 YAN Mengjie;LU Jianghao;SUN Xinkai;HUO Wenmin;MA Xinying;CUI Wenjing;HE Fang;ZHAO Linsen(Hebei Inatural BioTech Co,Ltd.,Shijiazhuang,Hebei 050000,China;不详)

机构地区：[1]河北一然生物科技有限公司,河北石家庄050000 [2]四川大学华西公共卫生学院营养与食品卫生及毒理学系,四川成都610000

出　　处：《中国微生态学杂志》2020年第12期1370-1374,共5页Chinese Journal of Microecology

基　　金：石家庄市科技型中小企业技术创新资金项目(17SCX03006)。

摘　　要：目的分离筛选出在维护口腔微生态平衡方面具有潜在益生特性的乳杆菌菌株。方法从健康志愿者的口腔样本中分离乳杆菌,采用生理生化和16S rDNA分子测序进行菌株鉴定,并检测其抑菌能力、凝集能力、表面疏水性以及对溶菌酶耐受性。结果筛选出1株鼠李糖乳杆菌LR863,对变形链球菌、戈登链球菌、牙龈卟啉单胞菌、具核梭杆菌和伴放线放线杆菌具有抑菌作用,经蛋白酶处理后其抑菌活性降低。同时鼠李糖乳杆菌LR863有较强的自凝集能力并对上述5株指示菌有共凝集效果,对二甲苯、氯仿和乙酸乙酯的疏水率依次为76.91%、87.46%和41.88%,能耐受2.0 mg/mL浓度的溶菌酶。结论鼠李糖乳杆菌LR863具有优良生物学特性,可作为口腔保健产品的候选益生菌株。Objective To evaluate the probiotic potential of Lactobacillus isolated from oral cavity and select candidates to be used as probiotic in oral health.Methods Lactobacillus strains were screened from saliva and dental plaque by using selective medium.The Lactobacillus strains were identified and examined for antimicrobial activity,co-aggregation ability,hydrophobicity and resistance to lysozyme.Results A strain of Lactobacillus rhamnosus named LR863 was obtained,which exhibited antimicrobial activitity against Streptococcus mutans,Streptococcus gordonii,Porphyromonas gingivalis,Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans.Its antimicrobial activity decreased after protease treatment.This strain also showed excellent autoaggregation ability and co-aggregation ability.The hydrophobicity rates with xylene,chloroform and ethyl acetate were 76.91%,87.46%and 41.88%respectively.Moreover,L.rhamnosus LR863 showed resistance to 2.0 mg/mL lysozyme.Conclusion L.rhamnosus LR863 exhibited a spectrum of probiotic properties,which may facilitate its applications in oral care products.

关 键 词：口腔健康 鼠李糖乳杆菌 抑菌能力 凝集能力 

分 类 号：R378[医药卫生—病原生物学]