miR-126在口腔鳞状细胞癌组织中的表达及其临床意义  

Expression of miR-126 in oral squamous cell carcinoma tissues and its clinical significance

作　　者：高永强 施鹏伟 刘晓晓 师文楷[3] 刘一鸣[2] GAO Yongqiang;SHI Pengwei;LIU Xiaoxiao;SHI Wenkai;LIU Yiming(Department of Oral Periodontal,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,Henan,China;Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,Henan,China;Department of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,Henan,China)

机构地区：[1]郑州大学第一附属医院口腔牙周科,河南郑州450000 [2]郑州大学第一附属医院口腔颌面外科,河南郑州450000 [3]郑州大学第一附属医院肝胆胰外科,河南郑州450000

出　　处：《中国肿瘤生物治疗杂志》2021年第1期48-54,共7页Chinese Journal of Cancer Biotherapy

基　　金：河南省高等学校重点科研资助项目(No.20B320019)。

摘　　要：目的:检测miR-126在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达,分析其表达与患者临床病理特征及预后的关系,探讨其过表达对Tca8113细胞恶性生物学行为的影响。方法:选取2016年6月至2018年6月在郑州大学第一附属医院手术治疗的62例OSCC患者的癌及癌旁组织标本,以及人舌鳞癌细胞株Tca8113和人口腔角质细胞株HOK,用qPCR法检测癌组织及细胞中miR-126的表达,分析miR-126表达与患者临床病理特征和预后的关系。利用脂质体转染技术,将miR-126 mimics、miR-NC质粒分别转染进Tca8113细胞,分别采用MTT法、流式细胞术及Trasnwell小室法检测细胞增殖、凋亡、迁移及侵袭能力,WB法检测凋亡、迁移和侵袭相关蛋白的表达水平。结果:miR-126在OSCC组织和Tca8113细胞中的表达水平明显低于癌旁组织和HOK细胞(均P<0.01)。miR-126表达与OSCC患者的TNM分期、淋巴结转移相关联(均P<0.05),miR-126高表达患者的总生存率显著高于低表达组(P<0.05)。转染miR-126 mimics后,Tca8113细胞的增殖、迁移及侵袭能力明显降低(P<0.05或P<0.01),凋亡率明显升高(P<0.01);细胞中Bcl-2、N-cadherin和vimentin表达水平明显降低(均P<0.01),Bax和E-cadherin表达水平明显升高(均P<0.01)。结论:miR-126在OSCC组织及Tca8113细胞中低表达,上调miR-126可抑制Tca8113细胞的增殖、迁移和侵袭能力,并促进细胞凋亡。Objective: To detect the expression of miR-126 in oral squamous cell carcinoma(OSCC) and to analyze its correlation with clinicopathological features and prognosis of patients, as well as to explore the effect of miR-126 over-expression on the malignant biological behaviors of Tca8113 cells. Methods: A total of 62 pairs of cancer and para-cancerous tissue specimens from OSCC patients who were surgically treated in the First Affiliated Hospital of Zhengzhou University from June 2016 to June 2018 were collected for this study;in addition, human tongue squamous carcinoma Tca8113 cell line and human mouth keratinocyte HOK cell line were also selected for this study. The expression of miR-126 in cancer tissues and cells was detected by qPCR, and the relationship between miR-126 expression and clinicopathological features and prognosis of the patients was analyzed. miR-126 mimics and miR-NC plasmids were respectively transfected into Tca8113 cells by liposome transfection technology. Cell proliferation, apoptosis, migration and invasion were detected by MTT method, Flow cytometry and Transwell chamber method, respectively;and the expressions of apoptosis, migration and invasion related proteins were detected by Western blotting. Results: The expression level of miR-126 in OSCC tissues and Tca8113 cells was significantly lower than that in para-cancerous tissues and HOK cells(all P<0.01). The expression of miR-126 was associated with TNM stage and lymph node metastasis(all P<0.05), and patients with high miR-126 expression had significantly better overall survival rate than patients with low expression(P<0.05). After transfection with miR-126 mimics, the cell proliferation, migration and invasion ability significantly decreased(P<0.05 or P<0.01) while the apoptosis rate significantly increased in Tca8113 cells(P<0.01), the expression levels of Bcl-2, N-cadherin and vimentin in Tca8113 cells significantly decreased(all P<0.01), and expression levels of Bax and E-cadherin significantly increased(all P<0.01). Conclusion: m

关 键 词：微小RNA-126 口腔鳞状细胞癌 TCA8113细胞 增殖 凋亡 侵袭 预后 

分 类 号：R739.85[医药卫生—肿瘤] R730.7[医药卫生—临床医学]