巨噬细胞刺激1在卵巢癌中的表达及作用研究  

Study on the expression and function of macrophage stimulating 1 in ovarian cancer

作　　者：陈佳 吴晓娟[1] 郭娟[2] 王亚冬[3] Chen Jia;Wu Xiaojuan;Guo Juan;Wang Yatlong(Department of Obstetrics and Gynecology,Chongqing Emergency Medical Center,Chongqing 400014,China;Department of Obstetrics and,Gynecology,the Fifth People's Hospital of Chongqing,Chongqing 400062,China;Department of Breast and Thyroid Surgery,Chongqing Traditional Chinese Medicine hospital,Chongqing 400021,China)

机构地区：[1]重庆市急救医疗中心妇产科,400014 [2]重庆市第五人民医院妇产科,400062 [3]重庆市中医院乳腺甲状腺科,400021

出　　处：《中华内分泌外科杂志》2021年第1期98-102,共5页Chinese Journal of Endocrine Surgery

基　　金：重庆市卫生和计划生育委员会资助项目(2015MSXM216)。

摘　　要：目的探讨巨噬细胞刺激1 (macrophage stimulating 1,MST1)在卵巢癌中的表达,与患者临床病理特征的关系及其可能的作用机制。方法收集2016年3月至2020年2月重庆市急救医疗中心和重庆市第五人民医院62例卵巢癌患者肿瘤组织和癌旁组织,采用实时定量逆转录(RT-qPCR)检测mRNA的表达,CCK8法检测细胞增殖能力,生物信息学软件筛选可与MST1相互作用的蛋白质。结果 MST1在卵巢癌组织中的表达低于癌旁组织[(0.52±0.12)vs(1.18±0.21)]。MST1表达水平与年龄无关,但与肿瘤大小[(0.46±0.12)vs(0.58±0.10),P=0.00]、TNM分期[(0.57±0.10)vs(0.43±0.12),P=0.00]及淋巴结转移显著相关[(0.47±0.14)vs(0.56±0.09),P=0.003]。过表达MST1可显著抑制卵巢癌细胞的增殖[MST1过表达组: 24 h(0.31±0.02)、48 h(0.44±0.03)、72 h(0.62±0.02);空白对照组:24 h(0.32±0.02)、48 h(0.55±0.02)、72 h(0.74±0.02);MST1空载组:24 h(0.32±0.03)、48 h(0.56±0.02)、72 h(0.77±0.02)]。过表达MST1可促进FOXO3的表达。MST1过表达组和对照组中FOXO3表达水平分别为[(0.61±0.04)vs(0.41±0.03)]。MST1通过上调FOXO3表达可抑制卵巢癌细胞增殖。结论 MST1表达水平与卵巢癌患者临床病理特征密切相关,MST1可能通过正调控FOXO3发挥抑癌作用。Objective To investigate the expression of MST1 in ovarian cancer(OV),its relationship with the clinicopathological characteristics,and the potential molecular mechanism.Methods 62 OV patients admitted to Chongqing Emergency Medical Center and the Fifth People's Hospital of Chongqing from Mar.2016 to Feb.2020 were selected.The expression levels of mRNA were detected by quantitative real-time polymerase chain reaction.[MST1 over-expression group:24 h:(0.31±0.02),48 h:(0.44±0.03),72 h:(0.62±0.02);Blank group:24 h:(0.32±0.02),(0.55±0.02),(0.74±0.02);MST1 empty vector group:24 h:(O.32±0.03),48 h:(0.56±0.02),72 h:(0.77±0.02)]Results The expression of MST1 was lower in OV than in adjacent tissues[(0.52±0.12)vs(1.18±0.21)].MST1 expression level was not related to age,but significantly correlated with the size of the tumors(0.46±0.12)vs(0.58±0.10),P=0.00,TNM(0.57±0.10)vs(0.43±0.12),P=0.00,TNM[(0.57±0.10)vs(0.43±0.12),P=0.00] and lymph node metastasis[(0.47±0.14)vs(0.56±0.09),P=0.003].Over-expression of MST1 obviously inhibited cellular proliferation in OV(MST1 over-expression group:24 h:0.31±0.02,48 h:0.44±0.03,72 h:0.62±0.02;blank group:24 h:0.32±0.02,0.55±0.02.0.74±0.02;empty vector group:24 h:0.32±0.03,48 h:0.56±0.02,72 h:0.77±0.02).MST1 over-expression could promote FOXO3 expression,,the expression level of FOXO3 in Mstl overexpression group and control group were[(0.61±0.04)vs(0.41±0.03)].MST1 inhibited proliferation of OV cells through upregulating the expression of FOXO3.Conclusions The expression of MST1 is closely related to the clinicopathological features of OV patients,and MST1 may restrain OV by positively regulating FOXO3 expression.

关 键 词：巨噬细胞刺激1 卵巢癌 FOXO3 

分 类 号：R73[医药卫生—肿瘤]