紫甘薯花色苷通过调控KTN1-AS1表达对肺癌细胞的增殖和凋亡的影响  

Effects of Purple Sweet Potato Anthocyanins on Proliferation and Apoptosis of Lung Cancer Cells by Regulating Expression of KTN1-AS1

作　　者：范智彦 刘金菊 FAN Zhi-yan;LIU Jin-ju(Linyi Third People's Hospital,Linyi 276004,China;Linyi Tumor Hospital,Linyi 276004,China)

机构地区：[1]临沂市第三人民医院,山东临沂276004 [2]临沂市肿瘤医院,山东临沂276004

出　　处：《食品与药品》2021年第1期10-16,共7页Food and Drug

基　　金：山东省自然科学基金(ZR2017PH045)。

摘　　要：目的探讨紫甘薯花色苷对肺癌细胞增殖和凋亡的影响及作用机制。方法体外培养肺癌A549细胞,分为对照组、紫甘薯花色苷不同剂量(200,400,800μg/ml)组、si-NC组、si-KTN1-AS1组、紫甘薯花色苷800μg/ml+pc DNA组、紫甘薯花色苷800μg/ml+pc DNA-KTN1-AS1组,细胞计数试剂盒-8(CCK-8)检测细胞增殖,流式细胞仪检测细胞凋亡,实时荧光定量PCR(RT-qPCR)检测细胞中KTN1-AS1表达水平,蛋白印迹法(Western blot)检测细胞周期蛋白D1(cyclin D1)、p21、B淋巴细胞瘤-2蛋白(Bcl-2)和B淋巴细胞瘤-2相关蛋白(Bax)表达水平。结果与对照组比较,紫甘薯花色苷不同剂量组细胞增殖抑制率、凋亡率及p21和Bax蛋白表达升高(P<0.05),cyclin D1和Bcl-2蛋白表达及KTN1-AS1表达降低(P<0.05),且呈剂量依赖性。与si-NC组比较,si-KTN1-AS1组细胞抑制率、凋亡率及p21和Bax蛋白表达升高(P<0.05),cyclin D1和Bcl-2蛋白表达降低(P<0.05)。与紫甘薯花色苷800μg/ml+pc DNA组比较,紫甘薯花色苷800μg/ml+pcDNA-KTN1-AS1组细胞抑制率、凋亡率及p21和Bax蛋白表达降低(P<0.05),KTN1-AS1及cyclin D1和Bcl-2蛋白表达升高(P<0.05)。结论紫甘薯花色苷可能通过下调KTN1-AS1表达抑制肺癌A549细胞增殖,并促进细胞凋亡。Objective To explore the effect and mechanism of purple sweet potato anthocyanins on lung cancer cell proliferation and apoptosis.Methods Lung cancer A549 cells were cultured in vitro and divided into control group,groups treated with different doses(200,400 and 800μg/ml)of purple sweet potato anthocyanins,si-NC group,siKTN1-AS1 group,purple sweet potato anthocyanins(800μg/ml)+pc DNA group,and purple sweet potato anthocyanins(800μg/ml)+pcDNA-KTN1-AS1 group.The cell proliferation was detected by CCK-8,the cell apoptosis was detected by flow cytometry,the expression level of KTN1-AS1 was detected by RT-qPCR,and the expression levels of cyclin D1,p21,Bcl-2 and Bax protein were detected by Western blot.Results Compared with the control group,the cell proliferation inhibition rate,apoptosis rate and the protein expression levels of p21 and Bax in groups treated by different doses of purple sweet potato anthocyanins increased(P<0.05),while the expression levels of cyclin D1 and Bcl-2 and KTN1-AS1 were reduced(P<0.05),and the effects were dose-dependent.Compared with si-NC group,the cell proliferation inhibition rate,apoptosis rate and the expression levels of p21 and Bax in the si-KTN1-AS1 group increased(P<0.05),while the expression levels of cyclin D1 and Bcl-2 were reduced(P<0.05).Compared with the purple sweet potato anthocyanins(800μg/ml)+pcDNA group,the cell proliferation inhibition rate,apoptosis rate and the expression levels of p21 and Bax in the purple sweet potato anthocyanins(800μg/ml)+pcDNA-KTN1-AS1 group were reduced(P<0.05),while the expression levels of KTN1-AS1 and cyclin D1 and Bcl-2 increased(P<0.05).Conclusion Purple sweet potato anthocyanins may inhibit the proliferation of lung cancer A549 cells and promote apoptosis by down-regulating the expression of KTN1-AS1.

关 键 词：紫甘薯花色苷 肺癌 KTN1-AS1 细胞增殖 凋亡 

分 类 号：R966[医药卫生—药理学]