肿瘤相关中性粒细胞诱导人粘液表皮样癌细胞上皮-间质转变和侵袭转移的功能及机制研究  

Functional and mechanistic study of tumor-associated neutrophils inducing epithelial-mesenchymal transformation,invasion and metastasis of human mucoepidermoid carcinoma cells

作　　者：黎哲昊 卜歆[2] 封琳 胡君璧 申亮亮[2] 关键[1] LI Zhehao;BU Xin;FENG Lin;HU Junbi;SHEN Liangliang;GUAN Jian(Department of Oral and Maxillofacial Surgery,Hospital of Stomatology,Jiamusi University,154002,China;Department of Biochemistry and Molecular Biology,the Fourth Military Medical University,Xi'an;Shaanxi University of Chinese Medicine,Xianyang;Department of Gastroenterology,The First Affiliated Hospital of Xi'an Jiaotong University)

机构地区：[1]佳木斯大学附属口腔医院颌面外科,154002 [2]第四军医大学基础医学院生物化学与分子生物学教研室 [3]陕西中医药大学 [4]西安交通大学第一附属医院消化内科

出　　处：《实用口腔医学杂志》2021年第1期5-9,共5页Journal of Practical Stomatology

基　　金：陕西省自然科学基础研究计划(编号:2019JQ-879);陕西省重点研发计划(编号:2020SF-081)。

摘　　要：目的:研究肿瘤相关中性粒细胞对人粘液表皮样癌MC3细胞上皮-间质转变(EMT)和侵袭转移过程调控的功能及机制。方法:分离培养C57小鼠骨髓中性粒细胞,Transwell检测肿瘤相关中性粒细胞对MC3侵袭和迁移的影响;Western印迹和Real-time PCR检测Vimentin等mRNA和蛋白表达;免疫荧光等检测Vimentin表达。结果:肿瘤相关中性粒细胞中TGF-β表达增加;MC3细胞与中性粒细胞共培养后Vimentin和Snail表达增加;裸鼠体内实验表明中性粒细胞浸润程度与Vimentin表达相关。结论:肿瘤相关中性粒细胞通过TGF-β诱导MC3细胞中Snail表达,并促进肿瘤侵袭和转移。Objective:To investigate the function and mechanism of tumor-associated neutrophils in regulating EMT,invasion and metastasis of MC3 cells.Methods:Neutrophils were isolated from bone marrow of C57 mice and in vitro co-cultured with MC cells.Transwell and wound healing assays were performed to detect the effects of tumor-associated neutrophils on MC3 cell invasion and migration.mRNA and protein expressions of Vimentin and Snail in MC3 cells were detected by Western blot and real-time PCR assays.The distribution and effects of neutrophils on Vimentin expression were detected by immunofluorescence staining assay.Results:TGF-βexpression was increased in tumor-associated neutrophils.Tumor-associated neutrophils increased the expression of Vimentin and Snail in MC3 cells.Metastatic tumors were infiltrated by neutrophils and associated with Vimentin expression.Conclusion:Tumor-associated neutrophils can induce Snail expression by secreting TGF-β,and subsequently promote tumor cell EMT,invasion and metastasis of MC3 cells.

关 键 词：人粘液表皮样癌 肿瘤相关中性粒细胞 上皮-间质转变 

分 类 号：R739.8[医药卫生—肿瘤]