荧光定量PCR方法检测肉制品中鳄鱼源性成分  

Detection of Crocodile Originin Meat by Fluorescence Quantitative PCR

作　　者：吴颖峰 董合磊 刘佳[1] 刘梅[1] 肖红梅[1] WU Yingfeng;DONG Helei;LIU Jia;LIU Mei;XIAO Hongmei(College of Food Science and Technology,Nanjing Agriculture University,Nanjing 210095,China)

机构地区：[1]南京农业大学食品与科技学院,江苏南京210095

出　　处：《食品与生物技术学报》2020年第11期81-87,共7页Journal of Food Science and Biotechnology

基　　金：江苏省农业科技自主创新资金项目(CX173038)。

摘　　要：针对鳄鱼色素细胞b基因序列设计特异性引物,建立一种快速、特异、灵敏的鳄鱼源性成分检测方法。以常见羊肉、牛肉、猪肉、鸡肉、鸭肉、鱼肉、虾肉为参考动物物种作特异性检测,并经检出限和灵敏度实验验证其可行性。结果表明:该方法能够有效对鳄鱼源性成分进行快速检测,具有较强的特异性,鳄鱼组分DNA的检出限可达0.001 ng/μL,灵敏度可达0.01%。通过加工肉制品的检测验证,该方法拥有特异性强、灵敏度高的特点,可以用于加工肉制品中鳄鱼源性成分的检测。A fast,specific and sensitive method for detection of crocodile-derived components was developed by designing specific primers on the cytb gene sequence of crocodiles.Mutton,beef,pork,chicken,duck,fish and shrimp were used as reference animals for specific detection.The feasibility of the method was verified by the detection limit and sensitivity test.The results showed that the method was effective in rapid detection of crocodile-derived components,with strong specificity,and the detection limit of DNA of crocodile-derived components was up to 0.001 ng/μL,and the sensitivity was up to 0.01%.The method has the characteristics of strong specificity and high sensitivity,which can be used for the detection of crocodile-derived components in processed meat products.

关 键 词：鳄鱼源性成分 CYTB 荧光定量PCR 检测 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]