基于便携式生物传感器设备的转基因现场快速分析研究  

Advances in portable biosensor devices for point-of-care test of genetically modified crops

作　　者：崔丹丹 翟杉杉 文璐珂 武玉花[1] 李俊[1] 李允静[1] 李晓飞[1] 朱莉[1] 高鸿飞 吴刚[1] CUI Dan-dan;ZHAI Shang-shang;WEN Lu-ke;WU Yu-hua;LI Jun;LI Yun-jing;LI Xiao-fei;ZHU Li;GAO Hong-fei;WU Gang(Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2021年第1期22-30,共9页Chinese Journal of Oil Crop Sciences

基　　金：转基因重大专项(2018ZX08012001-005);湖北省自然科学基金(2020CFB872);中央级科研院所基本科研业务费专项(1610172018012)。

摘　　要：现场快速检验(point of care test,POCT)是实现转基因基层监管必要的技术手段,在转基因成分检测中发挥着重要作用。生物传感器为转基因成分简单、快速和低成本定量分析提供了一个可行的技术方案。目前大量的生物传感器研究还处于方法学研究阶段,研究者需要着重将生物传感器技术与分析设备集成,开发小型化的便携式检测系统,从而满足POCT分析。本文回顾了近年来已开发的基于手持式设备的用于转基因POCT分析的微型生物传感器系统研究进展,提出了未来可用于转基因POCT分析的生物传感器的小型化策略,展望了小型化转基因生物传感器系统的发展趋势、发展前景和面临的挑战。Point-of-care test(POCT)is a necessary technology to implement the supervision of genetically modified(GM)crops in resource-limited settings,which plays an important role in GM organisms monitoring.Recently,biosensor provides a promising pathway for a simple,quick and affordable quantification of GM ingredients.So far plenty of studies have concentrated on the methodology development of biosensor,however its application has rarely been reported.The development of portable biosensors with desirable characteristics of integration and miniaturization is highly demand for POCT.This paper reviews recent advances in handheld device-assisted miniaturized biosensor systems for POCT of GM crops.Miniaturization strategies of biosensors for POCT of GM organisms in the future are then proposed.The trends,prospects and challenges of miniaturized biosensors for POCT are further described.

关 键 词：生物传感器 现场快速分析 转基因生物 手持式设备 小型化 

分 类 号：TS201[轻工技术与工程—食品科学] S188[轻工技术与工程—食品科学与工程]