转mEPSPS基因甘蓝型油菜对根际土壤细菌群落结构和多样性的影响  

Effect of transgenic Brassica napus with mEPSPS gene on diversity and structure of rhizosphere microbial communities

作　　者：杜坤[1] 王婷[1] 杨阳[1] 李金萍 王幼平[1] DU Kun;WANG Ting;YANG Yang;LI Jin-ping;WANG You-ping(College of Biosciences and Biotechnology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《中国油料作物学报》2021年第1期131-140,共10页Chinese Journal of Oil Crop Sciences

基　　金：转基因生物新品种培育重大专项(2018ZX08020001-012)。

摘　　要：转基因作物外源基因的表达是否会对土壤微生物生态系统产生不利影响是转基因作物环境安全评价中的重要一环,也关系到作物新品种的应用与推广。为此,本文采用第三代16S扩增子高通量测序技术,对转mEPSPS基因的甘蓝型油菜甲HX6及其亲本甲9707不同发育阶段的根际土壤细菌群落进行了比较分析。结果显示,样品中油菜根际的优势细菌主要有变形菌门、拟杆菌门、酸杆菌门、放线菌门等。在同一发育时期,转基因材料和对照根际细菌的Alpha多样性分析(如Shannon、Simpson指数),主坐标(PCoA)分析和MRPP分析均显示无显著差异。此外,还发现转mEPSPS基因油菜在花期根际中浮霉菌门的相对丰度显著低于对照,三个时期中均有少数细菌属的相对丰度也存在显著性差异。不同发育时期,油菜根际细菌的多样性和群落结构差异较大,尤其是苗期与另两个时期相比差异显著。总的来看,转mEPSPS基因甘蓝型油菜的基因转化事件并未对根际土壤的细菌群落产生显著的不利影响。但是,为了更加全面科学地评价其是否存在潜在的土壤微生物生态安全风险,还需要在较长时期内监测其动态变化过程。Evaluating possible negative effects of alien genes expressed in transgenic plants is essential in environmental safety assessment of genetically modified(GM)organisms.We compared bacterial communities in rhizosphere soil of Brassica napus overexpressing mEPSPS(JHX6)and the control line J9707.Results showed that Proteobacteria,Bacteroidetes,Acidobacteria and Actinobacteria were the dominant bacterias in the rhizosphere soil of B.napus.Neither alpha diversity analysis(e.g.Shannon and Simpson index),PCoA nor MRPP analysis had revealed significant differences between the same developmental stage of the GM rape and control line.Meanwhile,Planctomycetes was less enriched in rhizosphere soil of GM rape at flowering stage compared with that of wild type.The enrichments of few bacterial genera were significantly different at 3 developmental stages of the GM rape and wild type.The bacterial diversity and community structure were great difference among the 3 developmental stages,especially in comparison of seedling stage.In total,mEPSPS overexpression in B.napus plants caused no significant variation on bacterial community in rhizosphere soil.While long-term dynamic monitoring is still necessary.

关 键 词：mEPSPS基因 甘蓝型油菜 微生物多样性 群落结构 

分 类 号：S154.3[农业科学—土壤学] S565.4[农业科学—农业基础科学]