转录抑制共遏因子在肝癌中的表达及功能  

Expression and function of BCL6 corepressor in hepatocellular carcinoma

作　　者：张浩文 陈松 禹鹏飞 赵栓柱 刘攀 付强 胡明星 秦涛 Zhang Haowen;Chen Song;Yu Pengfei;Zhao Shuanzhu;Liu Pan;Fu Qiang;Hu Mingxing;Qin Tao(Department of Hepatobiliary Pancreatic Surgery,People′s Hospital of Zhengzhou University,Zhengzhou 450003,China;Translational Research Institute Integrative Research Hub,Henan Provincial People′s Hospital,Zhengzhou 450003,China)

机构地区：[1]郑州大学人民医院(河南省人民医院)肝胆胰腺外科,450003 [2]河南省人民医院转化医学中心,郑州450003

出　　处：《中华实验外科杂志》2021年第2期336-338,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金 (31671440);河南省科技厅科技攻关资助项目 (172102310099)。

摘　　要：目的观察人转录抑制共遏因子(BCOR)在肝癌组织及对应癌旁组织中的表达和其对肝癌细胞增殖、迁移的影响。方法蛋白质印迹法(Western blot)检测BCOR在2019年8月至2020年10月在郑州大学人民医院接受手术切除的60例肝癌组织及对应癌旁组织中的表达水平;将构建的BCOR过表达载体及空病毒载体,经慢病毒包装后稳定转染至肝癌细胞中,构成实验组与对照组,免疫印迹法检测其表达变化;细胞计数试剂盒(CCK-8)实验检测转染后肝癌细胞增殖能力;划痕实验检测转染后肝癌细胞迁移能力;Transwell实验检测转染后肝癌细胞侵袭能力。两组之间比较采用t检验。结果癌旁组织中BCOR表达高于肝癌组织(1.316±0.642)倍,差异有统计学意义(t=2.689,P<0.05)。Western blot结果表明BCOR在肝癌细胞中表达量低于人正常肝细胞(1.867±0.661)倍,差异有统计学意义(t=2.941,P<0.01)。转染后实验组肝癌细胞中BCOR表达水平高于对照组(3.038±1.007)倍,差异有统计学意义(t=7.246,P<0.01)。CCK-8实验结果显示0 h实验组吸光度(A)值(0.604±0.043)较对照组(0.619±0.014)差异无统计学意义(t=0.934,P>0.05)。24、48、72 h实验组A值(0.644±0.028、0.788±0.097、1.139±0.253)低于对照组(0.860±0.057、1.250±0.282、1.934±0.326),差异均有统计学意义(t=9.545、4.385、5.444,P<0.05)。划痕实验结果显示24、48、72 h实验组迁移细胞数目[(128.333±2.082)、(230.667±2.517)、(324.667±4.509)个]低于对照组[(161.333±4.933)、(415.667±1.528)、(686.667±8.505)个],差异均有统计学意义(t=10.675、108.844、65.134,P<0.05)。Transwell实验结果显示实验组穿孔细胞数目[(56.833±9.368)个]低于对照组[(207.333±27.267)个],差异有统计学意义(t=12.786,P<0.05)。结论BCOR在肝癌组织中表达下调,过表达BCOR后可抑制肝癌细胞的增殖、迁移能力。Objective To observe the expression of B-cell lymphoma 6(BCL6)corepressor(BCOR)in hepatocellular carcinoma(HCC)tissues and its influence on the proliferation and migration of HCC cell.Methods Western blotting was used to detect the expression of BCOR in 60 cases of HCC tissues and their adjacent normal tissues.The BCOR overexpression vector and empty vector were constructed and stably transfected into HCC cells by lentivirus packaging to form an experimental group and a control group respectively.The expression of BCOR was detected by immunoblotting assay.The proliferation of experimental group and control group after transfection was detected by cell counting kit-8(CCK-8)assay.The migration ability was detected by scratch test.The ability of invasion was detected by Transwell assay.The t test was used for comparison between groups.Results The expression level of BCOR in paracancer tissue was(1.316±0.642)times higher than that in HCC tissues and the difference was statistically significant(t=2.689,P<0.05).Western blotting results showed that BCOR expression in HCC cells was(1.867±0.661)times lower than that in normal human liver cells,and the difference was statistically significant(t=2.941,P<0.01).The expression level of BCOR in the experimental group was(3.038±1.007)times higher than that in the control group,and the difference was statistically significant(t=7.246,P<0.01).The CCK-8 assay showed that the absorbance value in the experimental group(0.604±0.043)had no significant change at 0 h compared with that in the control group(0.619±0.014),and the difference was not statistically significant(t=0.934,P>0.05).The absorbance values in the experimental group at 24,48 and 72 h were(0.644±0.028),(0.788±0.097)and(1.139±0.253)respectively,significantly lower than those in the control group[(0.860±0.057),(1.250±0.282)and(1.934±0.326),t=9.545,4.385 and 5.444,P<0.01].The scratch test showed that the number of migrating cells in the experimental group at 2448 and 72 h[(128.333±2.082),(230.667±2.517)and(324.

关 键 词：实验组 肝癌细胞增殖 肝癌组织 细胞数目 转录抑制 细胞计数 免疫印迹法 蛋白质印迹法 

分 类 号：R735.7[医药卫生—肿瘤]