基于数据库挖掘:TRIM44蛋白在结直肠癌中的表达差异分析  

Based on database mining:analysis of the differential expression of TRIM44 protein in colorectal cancer

作　　者：王鹏宇[1] 胡圣晨 陈浙南 王彦 姜莹 WANG Pengyu;HU Shengchen;CHEN Zhenan;WANG Yan;JIANG Ying(Mudanjiang Medical University,Mudanjiang,Heilongjiang 157000,China;The First Affiliated Hospital,University of South China,Hengyang,Hunan 421000,China)

机构地区：[1]牡丹江医学院,黑龙江省牡丹江市157000 [2]南华大学附属第一医院,湖南省衡阳市421000

出　　处：《中南医学科学杂志》2021年第1期12-19,共8页Medical Science Journal of Central South China

基　　金：黑龙江省大学生创新创业训练计划项目(201910229070);黑龙江省普通本科高等学校青年创新人才培养计划(2020-QC23);黑龙江省省属高等学校基本科研业务费科研项目(2018-KYYWFMY-0035)。

摘　　要：目的通过数据挖掘分析TRIM44在结直肠癌中的表达及意义。方法对TCGA和GEO数据库结直肠癌中TRIM44的数据进行R语言脚本分析,并通过GEPIA、TIMER等多个数据库进行了差异表达、生存分析、肿瘤纯度与免疫浸润的关系以及相关基因的共表达和GO与KEGG分析。结果TRIM44在结肠癌中有明显的差异表达(P<0.001),TRIM44高表达者预后较差(P<0.05)。TRIM44在直肠癌中无明显差异表达。GO和KEGG分析结果显示,TRIM44共表达基因富集于调节干细胞多能性信号传导途径等多个通路。进一步免疫浸润分析提示TRIM44与M 0和M 2免疫细胞基因有明显的相关性。结论TRIM44可通过作用于M 0和M 2细胞影响CCL2基因表达,进而对结肠癌的发生和发展产生调控作用。TRIM44有望成为结肠癌早期诊断和免疫治疗新靶点。Aim To analyze the expression and significance of TRIM44 in colorectal cancer by data-mining.Methods This article analyzes the data of TRIM44 in colorectal cancer in TCGA and GEO databases using R script analysis,and conducts differential expression,survival analysis,relationship between tumor cure and immune infiltration,as well as co-expression of related genes through GEPIA,TIMER and other databases and GO and KEGG analysis.Results The results showed that TRIM44 was significantly differentially expressed in colorectal cancer(P<0.001),and those with high expression of TRIM44 had a worse prognosis(P<0.05).There is no significant difference in the e xpression of TRIM44 in rectal cancer.GO and KEGG analysis results showed that TRIM44 co-expressed genes are e nriched in multiple pathways such as the regulation of stem cell pluripotency signaling pathways.Further analysis of i mmune infiltration indicated that TRIM44 has a significant correlation with M 0 and M 2 immune cell genes.Conclusion It is suggested that TRIM44 can affect the CCL2 expression pathway by acting on M 0 and M 2 cells,thereby regulating the occurrence and development of colorectal cancer.TRIM44 is expected to become a new target for early diagnosis and immunotherapy of colorectal cancer.

关 键 词：TRIM44 结直肠癌 信号通路 数据挖掘 巨噬细胞 

分 类 号：R735.37[医药卫生—肿瘤]