miR-365-3p抑制CDK-10表达对口腔鳞癌细胞增殖、凋亡和周期的影响  

miR-365-3p inhibits the expression of CDK-10 and affects the proliferation,apoptosis and cycle of oral squamous cell carcinoma

作　　者：刘倩峰[1] 庞超[2] 李庆星[1] 杨佩璇 LIU Qianfeng;PANG Chao;LI Qingxing;YANG Peixuan(Department of Stomatology,the First Hospital of Hebei Medical University,Shijiazhuang,Hebei 050031,China;Department of Pathology,the First Hospital of Hebei Medical University,Shijiazhuang,Hebei 050031,China)

机构地区：[1]河北医科大学第一医院口腔科,河北省石家庄市050031 [2]河北医科大学第一医院病理科,河北省石家庄市050031

出　　处：《中南医学科学杂志》2021年第1期35-40,共6页Medical Science Journal of Central South China

基　　金：河北省医学科学研究课题(20190447)。

摘　　要：目的探讨miR-365-3p通过周期素依赖性激酶-10(CDK-10)影响口腔鳞癌(OSCC)细胞增殖、凋亡与周期的机制。方法收集在本院就诊的40例口腔鳞癌患者肿瘤组织样本及癌旁正常组织样本,检测其miR-365-3p和CDK-10表达水平。随机将人舌鳞癌细胞系(CAL-27)分为空白组、对照组及miR-365-3p三组。对照组与miR-365-3p组分别转染miR-365-3p NC与miR-365-3p mimic,空白组不进行转染。采用实时荧光定量PCR(qRT-PCR)法检测miR-365-3p表达,CCK-8法及流式细胞术(FCM)检测细胞周期和细胞凋亡。qPCR和Western blot检测CDK-10的表达水平。结果相较于对照组与空白组,miR-365-3p在miR-365-3p组的表达水平显著升高(P<0.05),细胞增殖指数显著降低(P<0.05);细胞凋亡水平显著升高(P<0.05)。转染72 h后,相较于空白组与对照组,miR-365-3p组G1期比例显著降低(P<0.05),S期+G2期比例则显著增加(P<0.05)。转染48 h与72 h后,相较于空白组与对照组CDK-10蛋白在miR-365-3p组的相对表达量显著降低(P<0.05)。结论miR-365-3p在口腔鳞癌细胞中的高表达能够抑制CDK-10表达,从而抑制细胞增殖,调节细胞周期,促进细胞凋亡。Aim To explore and analyze the mechanism of miR-365-3p affecting the proliferation,apoptosis and cycle of oral squamous cell carcinoma cells through cyclin-dependent kinase-10(CDK-10).Methods Collect 40 patients with oral squamous cell carcinoma who were treated in our hospital,extract the patient’s tumor tissue samples and adjacent normal tissues,detect the expression of miR-365-3p and CDK-10,and randomly select the human tongue cancer cell lines(CAL-27)which were divided into three groups:blank group,control group and miR-365-3p.The control group and the miR-365-3p group were transfected with miR-365-3p NC and miR-365-3p mimic,respectively,and the blank group were not transfected.The expression of miR-365-3p were detected by PCR(qRT-PCR),Cell counting kit-8(CCK-8)and flow cytometry(FCM)were used to detect cell cycle and apoptosis.The expression of CDK-10 was detected by qPCR and Western blot.Results Compared with the control group and the blank group,the expression level of miR-365-3p in the miR-365-3p group was significantly higher(P<0.05),and the cell proliferation index was significantly lower(P<0.05);the apoptosis index was significantly increase(P<0.05).After 72 hours of transfection,compared with the blank group and the control group,the ratio of G1 phase in the miR-365-3p group was significantly reduced(P<0.05),and the ratio of S phase+G2 phase was significantly increased(P<0.05).The relative expression of CDK-10 protein in the experimental group were significantly lower than that in the control group and the blank group at 48 h and 72 h after transfection.Conclusion The high expression of miR-365-3p in oral scale cancer cells inhibits CDK-10 expression,thereby inhibiting cell proliferation,regulating cell cycles,and promoting apoptosis.

关 键 词：miR-365-3p 口腔鳞癌细胞 细胞周期素依赖性激酶-10 细胞增殖 细胞凋亡 

分 类 号：R739.8[医药卫生—肿瘤]