滴度及注射方式对2型腺相关病毒载体表达效果的影响  

Effects of viral dose and injection method on expression of adeno-associated viral vector serotype 2

作　　者：贾俊涛[1] 王昆[2] 潘登[3] 李强[2] 杜晨光[1,3] JIA Juntao;WANG Kun;PAN Deng;LI Qiang;DU Chenguang(Vocational and.Technical College,Inner Mongolia Agricultural University,Baotou,Inner Mongolia 014109,China;Institute of Grain and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050000,China;College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学职业技术学院,内蒙古包头014109 [2]河北省农林科学院粮油作物研究所,河北石家庄050000 [3]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2021年第2期236-239,314,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31660701);河北省农林科学院创新工程资助项目(4-04-03);河北省现代农业产业技术体系肉牛产业创新团队建设资助项目(HBCT20181302)。

摘　　要：由于腺相关病毒载体(adeno-associated viral vector,AAV)本身的侵染不具有神经元特异性,其在神经系统相关研究中会存在侵染范围不符合试验要求的情况,因此本试验拟研究不同滴度和注射方式对病毒侵染范围的影响。结果显示,在注射较高滴度的AAV2-CMV-EGFP(1.3×10^(11)mg/L)3周后,其侵染范围可由延髓中缝苍白核(raphe pallidus nucleus,RPa)区域延伸至下丘脑室旁核(paraventricular nucleus,PVN)区域;较低滴度(1.3×10^(8)mg/L)结合压力注射的方式可以在一定程度上限制AAV的侵染范围,并且载体携带的EGFP荧光基团在637 nm波段不具有明显的假阳性信号。In recent years,adeno-associated viral(AAV)has been widely used as a new and efficient research tool in the life science research.Because infection of adenovirus vector itself is not neuron-specific,there may be cases where the range of AAV infection does not meet the requirements of the study in nervous system.Therefore,this experiment intends to investigate the effect of different dosages of virus and injection methods on infection of the virus vector.The results showed that,after being injected with high dosage of AAV2-CMV-EGFP(1.3×10^(11)mg/L)for three weeks,the infection range could extend from the area of the raphe pallidus nucleus(RPa)in medulla to the paraventricular nucleus(PVN)in hypothalamus,whereas low dosage of vector(1.3×10^(8)mg/L)combined with pressure injection method can limit the range of AAV infection,and the EGFP carried by the vector has no obvious false positive signal at 637 nm.

关 键 词：腺相关病毒载体 立体定位注射 滴度 

分 类 号：S852.2[农业科学—基础兽医学] S852.65[农业科学—兽医学]