细胞DNA倍体联合Hsp90α、LDH诊断良恶性胸腔积液、腹水的临床价值  

Clinical value of cell DNA ploidy pattern combined with heat shock protein 90αand LDH in differential diagnosis of benign and malignant pleural and peritoneal effusion

作　　者：张姚[1] 杨丽琼[1] 叶敏[1] 吉彩霞 ZHANG Yao;YANG Liqiong;YE Min;JI Caixia(Department of Clinical Laboratory,Deyang People′s Hospital,Deyang,Sichuan 618000,China)

机构地区：[1]德阳市人民医院检验科,四川德阳618000

出　　处：《国际检验医学杂志》2021年第5期604-607,612,共5页International Journal of Laboratory Medicine

基　　金：四川省卫生和计划生育委员会科研课题(18PJ579)。

摘　　要：目的探讨细胞DNA倍体联合热休克蛋白90α(Hsp90α)、乳酸脱氢酶(LDH)诊断良恶性胸腔积液、腹水的临床价值。方法选择2019年2月至2020年2月该院收治的137例胸腔积液患者,根据胸腔积液性质分为胸腔积液良性组(n=53)与胸腔积液恶性组(n=84),比较两组胸腔积液LDH、Hsp90α水平及DNA倍体阳性率;该院同期收治的122例腹水患者,根据腹水性质分为腹水良性组(n=50)与腹水恶性组(n=72),比较两组腹水LDH、Hsp90α水平及DNA倍体阳性率。采用Spearman相关分析胸腔积液、腹水LDH、Hsp90α水平及DNA倍体阳性率与恶性胸腔积液、腹水的相关性,采用受试者工作特征曲线(ROC曲线)分析胸腔积液、腹水LDH、Hsp90α水平及DNA倍体阳性率对良恶性胸腔积液、腹水的鉴别诊断价值。结果与胸腔积液、腹水良性组比较,胸腔积液、腹水恶性组患者胸腔积液、腹水中LDH、Hsp90α水平及DNA倍体阳性率呈上升趋势(P<0.05);胸腔积液、腹水LDH、Hsp90α水平及DNA倍体阳性率与恶性胸腔积液、腹水均呈正相关(P<0.05);胸腔积液、腹水Hsp90α对良恶性胸腔积液的鉴别诊断价值明显优于胸腔积液、腹水LDH(P<0.05);胸腔积液、腹水DNA倍体阳性率对良恶性胸腔积液的鉴别诊断价值明显优于胸腔积液、腹水Hsp90α(P<0.05);胸腔积液、腹水LDH、Hsp90α水平及DNA倍体阳性率联合检测对良恶性胸腔积液、腹水的鉴别诊断价值明显优于各项单独检测(P<0.05)。结论胸腔积液、腹水LDH、Hsp90α水平及DNA倍体阳性率联合检测对良恶性胸腔积液、腹水鉴别诊断价值较高,具有较好的临床应用价值。Objective To investigate the clinical value of cell DNA ploidy pattern combined with heat shock protein 90α(Hsp90α)and lactic dehydrogenase(LDH)in differential diagnosis of benign and malignant pleural and peritoneal effusion.Methods A total of 137 patients with pleural effusion and 122 patients with peritoneal effusion treated in our hospital from February 2019 to February 2020 were enrolled,and divided into groups according to the differential diagnosis results,benign pleural effusion group(n=53),malignant pleural effusion group(n=84),benign peritoneal effusion group(n=50)and malignant peritoneal effusion group(n=72).LDH,Hsp90αand the positive rate of DNA ploidy were compared between the benign and malignant groups.Correlation between LDH,Hsp90αlevels and DNA ploidy positive rate and malignant pleural and peritoneal effusion was verified by spearman analysis.Then the receiver operating characteristic curve(ROC)was used to analyze the differential diagnosis value of LDH,Hsp90αlevels and DNA ploidy for benign and malignant pleural and peritoneal effusion.Results Compared with benign groups,the levels of LDH,Hsp90αand the positive rate of DNA ploidy in pleural and peritoneal effusion of malignant groups showed an upward trend(P<0.05).LDH and Hsp90αand the positive rate of DNA ploidy in pleural and peritoneal effusion were positively correlated with malignant pleural and peritoneal effusion(P<0.05).In the single test,DNA ploidy in pleural and peritoneal effusion had the highest differential diagnosis value in benign and malignant pleural and peritoneal effusion,followed by Hsp90αand LDH,with statistical difference(P<0.05),while the diagnostic value of combined detection of LDH,Hsp90αlevels and DNA ploidy positive rates in pleural and peritoneal effusion were better than that of single detection(P<0.05).Conclusion Combined detection of LDH,Hsp90αlevels and DNA ploidy positive rate in pleural and peritoneal effusion is of high value in the differential diagnosis of benign and malignant pleural and peritonea

关 键 词：DNA倍体 热休克蛋白90Α 乳酸脱氢酶 胸腔积液 腹水 

分 类 号：R730.43[医药卫生—肿瘤]