基质硒含量对棘托竹荪菌丝生长及其谷胱甘肽过氧化酶活性的影响  

Effects of Selenium Content in Culture Medium on the Growth of Dictyophora echinovolvata Mycelium and the Activity of Glutathione Perxidase

作　　者：王裕霞[1] 陈新宇 徐斌[1] 廖焕琴 潘文[1] 张卫华[1] 杨晓慧[1] 杨会肖[1] 朱报著[1] 徐放[1] Wang Yuxia;Chen Xinyu;Xu Bin;Liao Huanqin;Pan Wen;Zhang Weihua;Yang Xiaohui;Yang Huixiao;Zhu Baozhu;Xu Fang(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou 510520,China)

机构地区：[1]广东省森林病虫害生物防治重点实验室/广东省林业科学研究院,广州510520

出　　处：《世界竹藤通讯》2021年第1期20-23,共4页World Bamboo and Rattan

基　　金：广东省林业科技创新项目“竹林下富硒竹荪立体栽培技术研究”(2017KJCX008)。

摘　　要：通过菌袋培养棘托竹荪试验,对比了不同基质硒含量对菌丝体生长速度及其谷胱甘肽过氧化酶活性的影响。结果表明,采用改良的基质配方比用传统生产常规的木糠加麦麸配方的菌丝生长速度可增加15.9%,在传统配方基质培养下,菌丝体长至满袋的时间为85 d,而改良配方基质菌丝体满袋时间只需要70 d;用改良配方基质添加外源硒的含量在0.5~4.0 mg/kg时,可促进菌丝体的生长,最佳配方在1.5个月内菌丝体长至满袋;基质硒含量为1.0~2.0 mg/kg时,菌丝体生长最快,质量约1.8 kg的菌袋在培养时间为50 d时各个配方均能完全长满。在培养基质中添加适量的外源硒肥对提高棘托竹荪菌丝体先端谷胱甘肽过氧化物酶活性有显著作用。With the experiment on bag culturing of Dictyophora echinovolvata,we compares the effects of the different contents of selenium in culture medium on the growth rate of mycelium and the activity of its glutathione perxidase.The results show that the mycelium growth rate under the improved formulation is 15.9%higher than that under the traditional formulation of wood chaff and wheat bran.It takes more than 85 days for mycelium to grow to full bag under traditional formulation,while only 70 days under the improved formulation.The mycelium growth could be promoted when the added content of selenium is 0.5-4.0 mg/kg,and mycelium could grow to full bag within 1.5 months under the best formulation.The mycelium grows fastest when the added content of selenium is 1.0-2.0 mg/kg.The mycelium in the bag weighing 1.8 kg could grow to full bag in the completely about 50 days under each formulation.Applying appropriate amount of selenium fertilizer in the culture medium has a significant effect on improving the activity of glutathione perxidase of D.echinovolvata.

关 键 词：棘托竹荪 菌袋培养 富硒栽培 菌丝生长 抗氧化活性 

分 类 号：S646.8[农业科学—蔬菜学]