黄芪皂苷Ⅱ对STZ诱导的糖尿病大鼠的肾脏保护作用研究  

Renal Protective Effect of Astragaloside-Ⅱ on STZ-induced Diabetic Rats

作　　者：高崇婷 桂定坤[1,2] 汪年松[1] 谢玲 苏君 叶丹 GAO Chong-ting;GUI Ding-kun;WANG Niansong;XIE Ling;SU Jun;YE Dan(Department of Nephrology,Shanghai Jiaotong University Affiliated Sixth People's Hospital,Shanghai,200233,China;Department of nephrorheumatology,Shanghai Health Medical College Affiliated Sixth People's Hospital East Hospital,Shanghai,201306,China;Shanghai Ocean University,Shanghai,201306,China)

机构地区：[1]上海交通大学附属第六人民医院肾内科,上海200233 [2]上海健康医学院附属第六人民医院东院肾脏风湿科,上海201306 [3]上海海洋大学,上海201306

出　　处：《现代生物医学进展》2020年第23期4401-4406,4417,共7页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81774052,81573738)。

摘　　要：目的:研究黄芪皂苷Ⅱ(Astragaloside-Ⅱ,AS-Ⅱ)对链脲佐菌素(Streptozocin,STZ)诱导的糖尿病肾病(Diabetic nephrology,DN)大鼠肾脏的保护作用。方法:将8周龄雄性SD(Sprague-Dawley)大鼠随机选取5只作为正常对照组,其余大鼠予腹腔注射55 mg/kg剂量STZ建立糖尿病模型。造模成功的大鼠随机分为模型组与AS-Ⅱ治疗组,每组5只。AS-Ⅱ治疗组予AS-Ⅱ3.2mg/(kgod)连续口服灌胃治疗9周,同时正常对照组和模型组给予等量生理盐水溶液灌胃。第9周末,收集大鼠24 h尿液测定尿微量白蛋白浓度,留取肾脏组织观察肾脏病理改变并检测肾组织中足细胞裂孔隔膜蛋白Nephrin、WT1和caspase-3的表达。结果:与糖尿病模型组比较,AS-Ⅱ治疗组DN大鼠肾脏病理损伤明显改善,caspase-3表达明显减少,Nephrin和WT1表达增加,尿蛋白排泄减轻(P<0.05)。结论:AS-Ⅱ治疗可改善DN大鼠肾脏足细胞凋亡,降低尿蛋白,具有肾脏保护作用。Objective: To study the effects of Astragaloside-Ⅱ(AS-Ⅱ) on renal protection in streptozotocin(STZ) induced type 1 diabetic rats. Methods: Five male Sprague-Dawley(SD) rats aged 8 weeks were randomly selected as the normal control group. The rest of the rats were induced by intraperitoneal injection of STZ(55 mg/kg) to establish the model of type 1 diabetes rats. The diabetic rats were randomly divided into diabetic model group and AS-Ⅱ treatment group(n=5). AS-Ⅱ treatment group was given 3.2 mg/(kg·d)solution by gavage for 9 weeks. Normal control group and diabetes model group were given the same volume by gavage for 9 weeks. At the end of the 9 th week, urine albumin to creatinine ratio(ACR) and renal histopathology were examined, immunohistochemistry was used to detect the protein expression of Nephrin, WT1 and caspase-3. Results: Compared with diabetes model group, ACR and the renal histopathology was obviously improved, moreover, the expression of Nephrin and WT1 were up-regulated and the expression of caspase-3 were down-regulated in AS-Ⅱ treatment group. Conclusion: Therefore, AS-Ⅱ ameliorates podocyte apoptosis in STZ-induced type 1 diabetic rats, alleviate the pathological injury of the kidney, reduce ACR. has a protective effect on the kidney of STZ-induced type1 diabetic rats, which can delay the progression of diabetic nephropathy.

关 键 词：糖尿病肾病 黄芪皂苷Ⅱ 足细胞 凋亡 

分 类 号：R-33[医药卫生] R587.2