外周血中miRNAs表达水平对精神分裂症的诊断价值  

Diagnostic value of miRNA expression in peripheral blood for schizophrenia

作　　者：管娟 刘珺 秦文[1] GUAN Juan;LIU Jun;QIN Wen(Department of Psychiatry of No.15 Ward,Wuhan Mental Health Center,Wuhan 430014,Hubei,China)

机构地区：[1]武汉市精神卫生中心十五病区精神科,湖北武汉430014

出　　处：《川北医学院学报》2021年第2期237-241,共5页Journal of North Sichuan Medical College

摘　　要：目的:探讨外周血中miRNAs的表达水平对精神分裂症的诊断价值。方法:选取40例精神分裂症患者作为观察组,40例健康体检者作为对照组。实时定量聚合酶链反应(RT-qPCR)鉴定精神分裂症患者外周血液中miRNAs表达情况。通过受试者工作特征曲线(ROC曲线)评价miRNAs的诊断效能。采用可视化和集成发现数据库(DAVID)工具对miRNAs靶基因进行基因功能和京都基因和基因组百科全书(KEGG)通路富集分析。结果:两组受试对象外周血miR-30d-5p、miR-181b-3p、miR-652-5p、miR-193a-3p、miR-181b-5p、miR-346、miR-572、miR-7-5p、miR-564、miR-548d-3p和miR-30a-5p表达水平比较,差异无统计学意义(P>0.05);观察组miR-34a-5p、miR-449a和miR-22-3p表达水平高于对照组(P<0.05);miR-34a-5p、miR-449a和miR-22-3p的ROC曲线下面积(AUC)值分别为0.763,0.659和0.725,三者联合检测的AUC值为0.802。miR-34a-5p、miR-449a和miR-22-3p靶基因与突触结构和神经功能密切相关,三者共同靶基因SATB2和PER2在精神分裂症患者中异常表达(P<0.05)。结论:miR-34a-5p、miR-449a和miR-22-3p在精神分裂症中异常表达,三者及其靶基因结合有望作为精神分裂症诊断规范和治疗监测的生物标记物。Objective:To investigate the diagnostic value of miRNA expression in peripheral blood for schizophrenia.Methods:40 patients with schizophrenia were selected as the research objects and the same period health check-up 40 cases were selected as the control group.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to identify the expression of miRNAs in schizophrenia and the diagnostic efficacy of miRNAs was evaluated by receiver operating characteristic curve(ROC curve).Gene function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of miRNAs target genes were performed using Database for Annotation,Visualization and Integrated Discovery(DAVID)tool.Results:There was no significant difference in the expression levels of miR-30d-5p,miR-181b-3p,miR-652-5p,miR-193a-3p,miR-181b-5p,miR-346,miR-572,miR-7-5p,miR-564,miR-548d-3p and miR-30a-5p between the two groups(P>0.05).The expression levels of miR-34a-5p,miR-449a and miR-22-3p in the observation group were significantly higher than those in the control group(P<0.05).The area under ROC curve(AUC)values of miR-34a-5p,miR-449a and miR-22-3p were respectively 0.763,0.659 and 0.725,and the AUC value of the combination of them was 0.802.The target genes of miR-34a-5p,miR-449a and miR-22-3p were closely related to the synaptic structure and the function of nerve.The common target genes SATB2 and PER2 of three miRNA mentioned above were abnormally expressed in schizophrenia(P<0.05).Conclusion:The miR-34a-5p,miR-449a and miR-22-3p are abnormally expressed in schizophrenia.The combination of them and their target genes are expected to be biomarkers for diagnosis and treatment monitoring of schizophrenia.

关 键 词：精神分裂症 MIRNAS 靶基因 诊断 

分 类 号：R749.3[医药卫生—神经病学与精神病学]