微小核糖核酸-7调控表皮细胞生长因子受体对肝癌细胞增殖和转移的影响  

The effect of microRNA-7 regulating epidermal growth factor receptor on the proliferation and metastasis of hepatocellular carcinoma cells

作　　者：杨鹏 何洋 李成 王黎洲[1] 蒋天鹏[1] 许国辉[2] 周石[1] YANG Peng;HE Yang;LI Cheng;WANG Lizhou;JIANG Tianpeng;XU Guohui;ZHOU Shi(College of Medical Imaging,Guizhou Medical University,Guiyang,Guizhou Province 550004,China)

机构地区：[1]贵州医科大学影像学院,贵阳550004 [2]四川省肿瘤医院介入科

出　　处：《介入放射学杂志》2021年第1期43-47,共5页Journal of Interventional Radiology

基　　金：国家自然科学基金(81960328);四川省科技计划项目(2018JY0128)。

摘　　要：目的探讨微小核糖核酸(miR)-7是否通过靶向调控表皮细胞生长因子受体(EGFR)对人肝癌细胞侵袭和增殖产生影响。方法传代培养HepG2人肝癌细胞,脂质体转染miR-7后使其表达上调或下调。荧光定量聚合酶链反应检测转染效率。蛋白印迹法检测miR-7上调或下调后肝癌细胞EGFR表达变化。溴化噻唑蓝四氮唑(MTT)法检测miR-7上调或下调后肝癌细胞增殖能力。划痕试验检测miR-7上调或下调后肝癌细胞迁移能力。结果脂质体转染HepG2人肝癌细胞可控制miR-7表达,miR-7表达与肝癌细胞EGFR表达呈负相关。miR-7表达上调时,肝癌细胞增殖和迁移变慢;反之,miR-7表达下调时,肝癌细胞增殖和迁移变快。结论 miR-7靶向调节EGFR表达,其表达与肝癌细胞增殖和迁移呈负相关。miR-7可能是未来肝癌治疗新策略的潜在靶点。Objective To clarify the effect of microRNA-7(miR-7) on the invasion and proliferation of human hepatocellular carcinoma(HCC) cells through targeting regulation of epidermal growth factor receptor(EGFR). Methods Human HepG2 HCC cells were subcultured and miR-7 expression was up-regulated or down-regulated after miR-7 was transfected with liposome. The transfection efficiency of miR-7 expression was detected by real-time fluorescent quantitative polymerase chain reaction(PCR) method. The expressions of EGFR protein in HCC cells that were up-regulated or down-regulated by miR-7 were tested by Western Blot.MTT assay was used to test the proliferation ability of HCC cells that were up-regulated or down-regulated by miR-7. The migration ability of HCC cells that were up-regulated or down-regulated by miR-7 was checked by scratch test. Results The expression of miR-7 could be controlled by transfection of HepG2 HCC cells with liposomes,and the expression of miR-7 was negatively correlated with the expression of EGFR protein in HCC cells. When the expression of miR-7 was up-regulated,the proliferation and migration of HCC cells slowed down;on the contrary,when the expression of miR-7 was down-regulated,the proliferation and migration of HCC cells increased up. Conclusion MiR-7 can affect the expression of EGFR protein through targeting regulation,and the expression of EGFR bears a negative correlation with the proliferation and migration of HCC cells. Therefore,miR-7 may become a potential target for new therapeutic strategies for HCC in the future.(J Intervent Radiol,2021,30: 43-47)

关 键 词：肝癌 微小核糖核酸-7 表皮细胞生长因子受体 增殖 迁移 

分 类 号：R735.7[医药卫生—肿瘤]