miR-34b-5p上调抑制弥漫大B细胞淋巴瘤的侵袭及RhoA/ROCK信号通路  

Up-regulation of miR-34b-5p inhibits invasion of diffuse large B lymphoma and RhoA/ROCK signaling pathway

作　　者：李敬东[1] 韩效林[1] 杨翠[1] 高攀科 陈小双 梁勇会 Li Jingdong;Han Xiaolin;Yang Cui(Dept of Hematology,The First Affiliated Hospital of Xinxiang Medical College,Xinxiang 453100)

机构地区：[1]新乡医学院第一附属医院血液科,新乡453100

出　　处：《安徽医科大学学报》2021年第2期186-190,共5页Acta Universitatis Medicinalis Anhui

基　　金：河南省医学科技攻关计划项目(编号:201602152)。

摘　　要：目的研究miR-34b-5p过表达对弥漫大B细胞淋巴瘤的生长和运动以及RhoA/ROCK信号通路的影响。方法选择弥漫大B细胞淋巴瘤系中SU-DHL-4细胞进行研究。建立空白对照组、空载体转染组、miR-34b-5p转染组,采用qRT-PCR检测miR-34b-5p的表达量,采用菌落形成实验、流式细胞术和Transwell试验检测细胞增殖、凋亡及侵袭能力;采用Western blot检测E-cadherin、N-cadherin Vimentin、RhoA和ROCK蛋白的表达量。结果在转染miR-34b-5p的细胞内,miR-34b-5p的表达量上调。miR-34b-5p过表达能抑制弥漫大B细胞淋巴瘤SU-DHL-4细胞增殖,促进细胞凋亡,减轻细胞侵袭,抑制RhoA/ROCK信号通路。结论 miR-34b-5p过表达可以抑制弥漫大B细胞淋巴瘤的生长和运动以及RhoA/ROCK信号通路。Objective To study the effect of miR-34 b-5 p Up-regulation on the invasion of diffuse large B cell lymphoma and the RhoA/ROCK signaling pathway. Methods SU-DHL-4 cells were selected for study in diffuse large B lymphoma lines. A blank control group, an empty vector transfection group, and a miR-34 b-5 p transfection group were established. The expression of miR-34 b-5 p was detected by qRT-PCR. Cell proliferation, apoptosis and invasive were tested by colony formation assay, flow cytometry and Transwell test respectively. Western blot was used to detect the proteins expression levels of E-cadherin, N-cadherin Vimentin, RhoA and ROCK. Results miR-34 b-5 p expression was significantly up-regulated in cells transfected with miR-34 b-5 p. miR-34 b-5 p overexpression inhibited SU-DHL-4 cells proliferation, enhanced SU-DHL-4 cells apoptosis, reduced SU-DHL-4 cells invasion, and inhibited the RhoA/ROCK signaling pathway. Conclusion miR-34 b-5 p overexpression inhibits the growth and motility of diffuse large B cell lymphoma and the expression of RhoA/ROCK signaling pathway.

关 键 词：miR-34b-5p 弥漫大B细胞淋巴瘤 增殖 凋亡 侵袭 RhoA/ROCK信号通路 

分 类 号：R733.41[医药卫生—肿瘤]