酶联免疫法快速检测食品中重金属含量的研究进展  

Research Progress in Rapid Detection of Heavy Metals in Food by Enzyme-linked Immunosorbent Assay

作　　者：王莹[1] 王华[2] 贾纪萍[1] 丁宁[1] WANG Ying;WANG Hua;JIA Ji-ping(Department of Pharmacy,Jiangsu Animal Husbandry and Veterinary Science College,Taizhou,Jiangsu 225300;School of Pharmaceutical Sciences,Taizhou Institute of Sci.&Tech.,NJUST.,Taizhou,Jiangsu 225300)

机构地区：[1]江苏农牧科技职业学院动物药学院,江苏泰州225300 [2]南京理工大学泰州科技学院药学院,江苏泰州225300

出　　处：《安徽农业科学》2021年第5期10-13,共4页Journal of Anhui Agricultural Sciences

基　　金：2019年泰州市科技支撑计划社会发展(指导性)项目。

摘　　要：重金属超标是食品安全的重要问题之一,因此,需通过严格的监测,确保食品中的重金属含量在限量范围之内。传统的重金属检测方法灵敏度高、重复性好,但操作较为复杂,检测费用高,不适用于大量样本的快速筛选。近年来,随着酶联免疫检测技术的快速发展,酶联免疫(ELISA)法已广泛应用于食品中重金属的检测。从样品前处理、重金属人工抗原及特异性抗体的制备、ELISA法的选择三个方面介绍了该法在重金属检测方面的最新研究成果,并对ELISA法的发展进行了展望。Excessive heavy metal is one of the important problems of food safety.Therefore,strict monitoring is needed to ensure that the content of heavy metals in food is within the limit.The traditional heavy metal detection method has high sensitivity and repeatability,but its operation is complex and the detection cost is high,so it is not suitable for rapid screening of a large number of samples.In recent years,with the rapid development of enzyme-linked immunosorbent assay,enzyme-linked immunosorbent assay(ELISA)has been widely used in the detection of heavy metals in food.We mainly introduced the latest research results in heavy metal detection from three aspects:sample pretreatment,preparation of heavy metal artificial antigen and specific antibody,and selection of ELISA,and prospected the development of ELISA.

关 键 词：酶联免疫 重金属 抗原 抗体 

分 类 号：TS207.51[轻工技术与工程—食品科学]